EP 0563320 A4 19931118 - DETECTING CIGUATOXIN IN SERUM USING STICK ENZYME IMMUNOASSAY
Title (en)
DETECTING CIGUATOXIN IN SERUM USING STICK ENZYME IMMUNOASSAY
Publication
Application
Priority
US 63216590 A 19901221
Abstract (en)
[origin: WO9211385A1] The present invention relates to a method for detecting the presence of ciguatoxin or related polyether marine toxin in human serum. The steps for detecting the ciguatoxin comprise isolating a serum sample, attaching and fixing the ciguatoxin to a support, and rinsing the resultant toxin-support complex in a buffer to remove unbound toxin and to remove any residual fixer. The presence of ciguatoxin is detected by binding anti-toxin, an antibody against the ciguatoxin conjugated to an enzyme, to the toxin-support complex to form a toxin-antitoxin-enzyme complex. The enzyme of the toxin-anti-toxin-enzyme complex is assayed, and the amount of product formed by the enzyme is then quantitated. A kit for conducting the assay is also described.
IPC 1-7
IPC 8 full level
C07K 16/18 (2006.01); C12Q 1/28 (2006.01); G01N 33/53 (2006.01); G01N 33/548 (2006.01)
CPC (source: EP)
C07K 16/18 (2013.01); G01N 33/5308 (2013.01); G01N 33/548 (2013.01)
Citation (search report)
See references of WO 9211385A1
Designated contracting state (EPC)
AT BE DE DK FR GB IT LU NL SE
DOCDB simple family (publication)
WO 9211385 A1 19920709; AU 9177991 A 19920722; BR 9107179 A 19940208; CA 2098847 A1 19920622; EP 0563320 A1 19931006; EP 0563320 A4 19931118; JP H06511311 A 19941215
DOCDB simple family (application)
US 9109316 W 19911206; AU 9177991 A 19911206; BR 9107179 A 19911206; CA 2098847 A 19911206; EP 92904300 A 19911206; JP 50431092 A 19911206