EP 0564539 A4 19960306 - SUSTAINED AND CONTINUOUS PRODUCTION OF HIGH TITERS OF RECOMBINANT VIRAL VECTORS AND TRANSDUCED TARGET CELLS FOR USE IN GENE THERAPY
Title (en)
SUSTAINED AND CONTINUOUS PRODUCTION OF HIGH TITERS OF RECOMBINANT VIRAL VECTORS AND TRANSDUCED TARGET CELLS FOR USE IN GENE THERAPY
Publication
Application
Priority
US 62700890 A 19901213
Abstract (en)
[origin: WO9210564A1] This invention provides improved methods for producing high titers of recombinant viral vectors by culturing producer cells in a hollow fiber bioreactor. The titer of virus in the extra fiber space (EFS) of the hollow fiber bioreactor is sufficient to transduce target cells at a multiplicity of infection sufficient to render the transduced target cells useful for genetic therapy. In a preferred embodiment, target cells are transduced with the EFS medium from a hollow fiber bioreactor in which a producer cell line, which releases packaged retroviral vectors into the EFS, has been cultured. Lymphocytes derived from an adenosine deaminase (ADA)-deficient individual that have been transduced with EFS medium that contains retroviral vectors that include RNA that encodes ADA, express ADA at a rate comparable to that of cells from an individual who does not have ADA deficiency.
IPC 1-7
IPC 8 full level
C12M 1/00 (2006.01); C12M 3/06 (2006.01); C12N 5/10 (2006.01); C12N 7/00 (2006.01); C12N 9/26 (2006.01); C12N 9/78 (2006.01); C12N 15/09 (2006.01); C12N 15/867 (2006.01); A61K 48/00 (2006.01); C12R 1/92 (2006.01)
CPC (source: EP)
C12M 23/58 (2013.01); C12M 25/10 (2013.01); C12M 29/10 (2013.01); C12M 29/16 (2013.01); C12N 7/00 (2013.01); C12N 9/78 (2013.01); C12N 15/86 (2013.01); A61K 48/00 (2013.01); C12N 2740/14021 (2013.01); C12N 2740/14043 (2013.01)
Citation (search report)
- [DA] WO 9002171 A1 19900308 - CELLCO ADVANCED BIOREACTORS IN [US]
- [A] FR 2236004 A1 19750131 - MONSANTO CO [US]
- [DA] R.A. HOCK ET AL.: "Expression of human adenosine deaminase from various strong promoters after gene transfer into human hematopoietic cell lines", BLOOD, vol. 74, no. 2, 1 August 1989 (1989-08-01), SAUNDERS, DULUTH, NEW YORK , US, pages 876 - 881
- See references of WO 9210564A1
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IT LI LU MC NL SE
DOCDB simple family (publication)
WO 9210564 A1 19920625; AU 650711 B2 19940630; AU 9124691 A 19920708; CA 2098510 A1 19920614; EP 0564539 A1 19931013; EP 0564539 A4 19960306; JP H06500927 A 19940127
DOCDB simple family (application)
US 9109069 W 19911210; AU 9124691 A 19911210; CA 2098510 A 19911210; EP 92902297 A 19911210; JP 50269692 A 19911210