EP 0585983 A2 19940309 - Nucleotide vector comprising a translation inhibition factor gene, and IRES region and a desired gene.
Title (en)
Nucleotide vector comprising a translation inhibition factor gene, and IRES region and a desired gene.
Title (de)
Nukleotid-Vektor, enthaltend ein Gen für einen Traduktion inhibierenden Faktor, ein IRES-Gebiet und ein gewünschtes Gen.
Title (fr)
Vecteur nucléotidique comportant au gène de facteur inhibant la traduction, un IRES et un gène d'intérêt.
Publication
Application
Priority
IL 10240492 A 19920703
Abstract (en)
1. A method for the in vitro production of a gene product, comprises: (a) preparing one or more recombinant nucleotide vector(s) capable of eukaryotic cell transfection, collectively containing 1) a gene encoding an eukaryotic translation inhibition factor of an eukaryotic translation factor (ETF), 2) at least one internal ribosome entry site (IRES) region, and 3) at least one gene encoding a polypeptide which it is desired to produce; (b) transfecting a eukaryotic cell culture with the said vector; (c) keeping the transfected cell culture under conditions suitable to promote cell activity; and (d) recovering the desired gene product from the said culture. <IMAGE>
IPC 1-7
IPC 8 full level
A61K 48/00 (2006.01); C07K 14/105 (2006.01); C07K 14/47 (2006.01); C07K 14/61 (2006.01); C12N 5/10 (2006.01); C12N 9/16 (2006.01); C12N 15/09 (2006.01); C12N 15/85 (2006.01); C12P 21/00 (2006.01); A61K 39/00 (2006.01); C12R 1/91 (2006.01)
CPC (source: EP)
C07K 14/005 (2013.01); C07K 14/47 (2013.01); C07K 14/61 (2013.01); C12N 9/16 (2013.01); C12N 15/85 (2013.01); A61K 39/00 (2013.01); C12N 2770/32622 (2013.01); C12N 2830/002 (2013.01); C12N 2840/203 (2013.01); C12N 2840/85 (2013.01); Y02A 50/30 (2018.01)
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
EP 0585983 A2 19940309; EP 0585983 A3 19960110; IL 102404 A0 19930114; JP H0678784 A 19940322
DOCDB simple family (application)
EP 93201932 A 19930701; IL 10240492 A 19920703; JP 16346193 A 19930701