Global Patent Index - EP 0602044 A4

EP 0602044 A4 19941005 - A CDNA CLONE ENCODING A HUMAN NOREPINEPRHINE TRANSPORTER.

Title (en)

A CDNA CLONE ENCODING A HUMAN NOREPINEPRHINE TRANSPORTER.

Publication

EP 0602044 A4 19941005 (EN)

Application

EP 92910856 A 19920220

Priority

  • US 9201376 W 19920220
  • US 67698091 A 19910328

Abstract (en)

[origin: WO9217568A1] A cDNA encoding human norepinephrine transporter isolated from human neuroblastoma cells is disclosed. Determination of the nucleotide sequence thereof permitted a putative amino-acid sequence of the transporter protein to be determined. Mammalian cells transfected with the cDNA produced an mRNA species similar in size to that produced by norepinephrine-producing neurons. Such transfected cells also became capable of norepinephrine uptake. The uptake was inhibitable by various uptake-inhibiting drugs, including cocaine, to a degree similar to the effect of such drugs on noradrenergic neurons. The cloned cDNA makes possible well-controlled studies of transporter function in non-neuronal cells without the obfuscating influence of other transporters in the same cells. Such studies include the relative effects of various drugs such as antidepressants.

IPC 1-7

C12N 15/12

IPC 8 full level

C12N 15/09 (2006.01); C07K 14/435 (2006.01); C07K 14/705 (2006.01); C12N 5/00 (2006.01); C12N 5/10 (2006.01); C12N 15/12 (2006.01); C12N 15/79 (2006.01); C12P 21/02 (2006.01); C12R 1/91 (2006.01)

IPC 8 main group level

C07K (2006.01)

CPC (source: EP)

C07K 14/70571 (2013.01)

Citation (search report)

  • [X] NELSON, H. ET AL.;: "Cloning of the human brain GABA transporter", FEBS LETTERS., vol. 269, no. 1, August 1990 (1990-08-01), AMSTERDAM NL, pages 181 - 184
  • [A] COPPENEUR D;LINGEN B;SANDERS G;DABAUVALLE MC;BONISCH H;: "Expression of the neuronal noradrenaline transporter in Xenopus laevis oocytes.", NAUNYN-SCHMIEDEBERG'S ARCHIVES OF PHARMACOLOGY, vol. 343, no. 3, March 1991 (1991-03-01), SPRINGER-VERLAG, pages 334 - 336
  • [A] BONISCH H;MARTINY-BARON G;BLUM B;MICHAEL-HEPP J;: "Biochemical characterization and purification of the neuronal sodium-dependent noradrenaline transporter.", J NEURAL TRANSM SUPPL, vol. 32, 1990, pages 413 - 419
  • See references of WO 9217568A1

Designated contracting state (EPC)

AT BE CH DE DK FR GB IT LI LU NL SE

DOCDB simple family (publication)

WO 9217568 A1 19921015; AU 1774292 A 19921102; CA 2106190 A1 19920929; DK 107593 A 19930923; DK 107593 D0 19930923; EP 0602044 A1 19940622; EP 0602044 A4 19941005; FI 934234 A0 19930927; FI 934234 A 19930927; JP H07505040 A 19950608; NO 933378 D0 19930923; NO 933378 L 19930928

DOCDB simple family (application)

US 9201376 W 19920220; AU 1774292 A 19920220; CA 2106190 A 19920220; DK 107593 A 19930923; EP 92910856 A 19920220; FI 934234 A 19930927; JP 50993492 A 19920220; NO 933378 A 19930923