EP 0620861 A4 19950719 - RESTRICTION AMPLIFICATION ASSAY.
Title (en)
RESTRICTION AMPLIFICATION ASSAY.
Title (de)
RESTRIKTIONSAMPLIFIKATIONSTESTVERFAHREN.
Title (fr)
DOSAGE PAR AMPLIFICATION PAR ENZYME DE RESTRICTION.
Publication
Application
Priority
- US 9209606 W 19921112
- US 79017491 A 19911112
Abstract (en)
[origin: WO9310264A1] The present invention relates to a method, reagent and kit for the the determination of the presence of target nucleotide sequences by restriction amplification. In the process to detect nucleic acid sequences a target molecule containing a specific restriction site is hybridized with a labeled probe containing a sequence homologous to at least 28 bases of the target molecule. The probe is cleaved with a restriction enzyme that releases the probe for detection if the probe hybridizes to the specific target. Thus, the cleaved probe constantly regenerates and is highly detectable if the target sequence is present in the assay.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C07H 21/04 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP)
C12Q 1/682 (2013.01); C12Q 1/6823 (2013.01); C12Q 1/683 (2013.01)
Citation (search report)
- [A] EP 0455517 A1 19911106 - ONCOR INC [US]
- [A] EP 0234781 A2 19870902 - SZYBALSKI WACLAW
- See references of WO 9310264A1
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL SE
DOCDB simple family (publication)
WO 9310264 A1 19930527; CA 2122734 A1 19930527; EP 0620861 A1 19941026; EP 0620861 A4 19950719; JP H07505760 A 19950629
DOCDB simple family (application)
US 9209606 W 19921112; CA 2122734 A 19921112; EP 92924356 A 19921112; JP 50934293 A 19921112