EP 0634640 A1 19950118 - Method of counting reticulocytes.
Title (en)
Method of counting reticulocytes.
Title (de)
Verfahren zum Zählen von Reticulozyten.
Title (fr)
Procédé de comptage de réticulocytes.
Publication
Application
Priority
JP 12665193 A 19930528
Abstract (en)
Asymmetric cyanine dyes (and their dimers) of generic formula: A-(CH=CH)n-CH=B where A IDENTICAL 3-methyl-benzoxazolium or benzthiazolium iodide, B IDENTICAL 4-(N-trimethylaminopropyl)-pyridinium or quinolinium iodide, and n = 0 or 1 are used as fluorescent stains for staining reticulocytes in flow cytometric. A typical example is the thiazole orange dimer, TOTO-3. By suitable choice of components, the absorption maximum of the dye-RNA complex can be adjusted to allow the use of cheap, compact laser sources, e.g. Hc/Nc or laser diodes.
IPC 1-7
IPC 8 full level
C09B 23/02 (2006.01); G01N 15/14 (2006.01); G01N 33/80 (2006.01); G01N 1/30 (2006.01)
CPC (source: EP US)
C09B 23/02 (2013.01 - EP US); G01N 15/1456 (2013.01 - EP US); G01N 33/80 (2013.01 - EP US); G01N 2001/302 (2013.01 - EP US); G01N 2021/6439 (2013.01 - EP US); Y10S 436/80 (2013.01 - EP US); Y10T 436/101666 (2015.01 - EP US)
Citation (search report)
- [XAY] WO 9306482 A1 19930401 - MOLECULAR PROBES INC [US]
- [YA] US 4883867 A 19891128 - LEE LINDA G [US], et al
- [DA] US 4325706 A 19820420 - GERSHMAN RUSSELL J, et al
- [E] US 5321130 A 19940614 - YUE STEPHEN T [US], et al
- [YA] DATABASE WPI Section Ch Week 9325, Derwent World Patents Index; Class B04, AN 93-199619
- [A] RYE S R ET AL:: "Stable fluorescent complexes of double-stranded DNA with bis-intercalating asymmetric cyanine dyes: properties and applications", NUCLEIC ACIDS RESEARCH, vol. 20, no. 11, 1992, OXFORD GB, pages 2803 - 2812, XP002911844
- [PA] DATABASE WPI Section Ch Week 9348, Derwent World Patents Index; Class B02, AN 93-383255
Designated contracting state (EPC)
DE ES FR GB IT
DOCDB simple family (publication)
DOCDB simple family (application)
EP 94108238 A 19940527; US 24957694 A 19940526