Title (en)

Alcohol de hydrogenase, DNA encoding it, preparation and method of preparing optically active alcohols.

Title (de)

Alkohol-Dehydrogenase, dafür kodierende DNA, Herstellung und Verfahren zur Herstellung von optisch-aktiven Alkoholen.

Title (fr)

Déhydrogénase d'alcool, DNA, codant pour celle-ci, préparation et méthode de préparation d'alcools optiquement actifs.

Publication

EP 0645453 A3 19970326 (EN)

Application

EP 94115138 A 19940926

Priority

• JP 26164993 A 19930924
• JP 33719193 A 19931228
• JP 18130894 A 19940802

Abstract (en)

[origin: EP0645453A2] [Object] The present invention provides a novel secondary alcohol dehydrogenase useful for the synthesis of optically active alcohol and DNA encoding said enzyme. A microorganism belonging to genus Candida was found to produce a novel secondary alcohol dehydrogenase with a high stereochemical specificity. Using said enzyme, optically active alcohols were prepared, and by cloning of DNA encoding said enzyme, the base sequence of said DNA was determined. By providing a novel secondary alcohol dehydrogenase with a high stereochemical specificity and the gene encoding said enzyme, an efficient production of optically active alcohols became possible. <IMAGE>

IPC 1-7

C12N 15/53; C12N 9/04; C12N 1/21; C12N 15/70; C12P 7/24; C12P 7/04; C12P 41/00

IPC 8 full level

C12N 15/09 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 9/04 (2006.01); C12N 15/00 (2006.01); C12N 15/53 (2006.01); C12P 7/04 (2006.01); C12P 7/24 (2006.01); C12P 7/26 (2006.01); C12P 41/00 (2006.01); C12R 1/19 (2006.01); C12R 1/72 (2006.01)

CPC (source: EP US)

C12N 9/0006 (2013.01 - EP US); C12P 7/04 (2013.01 - EP US); C12P 7/24 (2013.01 - EP US); C12P 41/002 (2013.01 - EP US)

Citation (search report)

• [X] WO 9318138 A1 19930916 - FORSCHUNGSZENTRUM JUELICH GMBH [DE]
• [DX] DAVID W. GREEN ET AL.: "Inversion of the substrate specificity of yeast alcohol dehydrogenase", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 268, no. 11, 15 April 1993 (1993-04-15), MD US, pages 7792 - 7798, XP002024200
• [DA] CURT W. BRADSHAW ET AL.: "A Pseudomonas sp. alcohol dehydrogenase with broad substrate specificity and unusual stereospecificity for organic synthesis", JOURNAL OF ORGANIC CHEMISTRY, vol. 57, no. 5, 28 February 1992 (1992-02-28), EASTON US, pages 1526 - 1532, XP002024201

Designated contracting state (EPC)

DE FR GB IT NL

DOCDB simple family (publication)

EP 0645453 A2 19950329; EP 0645453 A3 19970326; EP 0645453 B1 20041124; DE 69434148 D1 20041230; DE 69434148 T2 20060216; JP 3574682 B2 20041006; JP H07231785 A 19950905; US 5763236 A 19980609; US 6255092 B1 20010703

DOCDB simple family (application)

EP 94115138 A 19940926; DE 69434148 T 19940926; JP 18130894 A 19940802; US 71325496 A 19960912; US 85576797 A 19970611