EP 0646173 A4 19970226 - REAGENT FOR AGGLUTINATION ASSAYS.
Title (en)
REAGENT FOR AGGLUTINATION ASSAYS.
Title (de)
REAGENZ ZUR MESSUNG DER AGGLUTINATION.
Title (fr)
REACTIF POUR DOSAGES D'AGGLUTINATION.
Publication
Application
Priority
- AU 9300228 W 19930519
- AU PL255192 A 19920522
Abstract (en)
[origin: WO9324630A1] The invention provides a bifunctional recombinant protein comprising a particle-binding antibody or antibody fragment (PBM), and an analyte-binding moiety or molecule (ABM). Preferably, the particle-binding antibody or antibody fragment is an erythrocyte-binding antibody or antibody fragment (EBM), and/or the ABM is selected from the group consisting of an antigenic peptide from an immunodominant region of an env protein of HIV-1 or HIV-2, a gag protein of HIV-1 or HIV-2, and an immunodominant region of the surface antigen of Hepatitis B. Alternatively, the ABM is a single chain Fv region of an antibody directed against an antigen selected from the group consisting of Hepatitis B surface antigen, D-dimer and canine heartworm antigen. In a particularly preferred embodiment, the EBM is a single chain Fv region of an anti-erythrocyte antibody, such as an anti-glycophorin antibody. The protein of the invention is particularly suitable for use in agglutination immunoassays. Methods for producing the protein of the invention, DNA sequences, and assay methods and reagent kits therefor are also provided.
IPC 1-7
C12N 15/13; C12N 15/70; C07K 15/12; C07K 15/28; G01N 33/563; G01N 33/577
IPC 8 full level
C07K 14/155 (2006.01); C07K 14/16 (2006.01); C07K 14/47 (2006.01); C07K 16/00 (2006.01); C07K 19/00 (2006.01); C12N 1/21 (2006.01); C12N 15/09 (2006.01); C12N 15/13 (2006.01); C12N 15/62 (2006.01); C12P 21/00 (2006.01); G01N 33/531 (2006.01); G01N 33/68 (2006.01); C12R 1/19 (2006.01)
CPC (source: EP)
C07K 14/005 (2013.01); C07K 14/47 (2013.01); C12N 15/62 (2013.01); G01N 33/531 (2013.01); G01N 33/6857 (2013.01); C07K 2319/00 (2013.01); C12N 2740/16122 (2013.01)
Citation (search report)
- [X] WO 8805913 A1 19880811 - CYTOSIGNET INC [US]
- [A] EP 0131424 A2 19850116 - NAT RES DEV [GB]
- [X] HILLYARD C. J. ET AL.,: "Disease diagnosis in two minutes", TODAY'S LIFE SCIENCE, vol. 3, - 1991, pages 52 - 60, XP000615338
- [X] KEMP B. E. ET AL.,: "Autologous red cell agglutination assay for hiv-1 antibodies: simplified test whole blood", SCIENCE, vol. 241, no. 4871, 9 September 1988 (1988-09-09), pages 1352 - 1354, XP000615341
- See references of WO 9324630A1
Designated contracting state (EPC)
CH DE FR GB IT LI
DOCDB simple family (publication)
WO 9324630 A1 19931209; EP 0646173 A1 19950405; EP 0646173 A4 19970226; JP H07506727 A 19950727; ZA 933552 B 19931209
DOCDB simple family (application)
AU 9300228 W 19930519; EP 93909677 A 19930519; JP 50001294 A 19930519; ZA 933552 A 19930521