EP 0649474 A4 19960403 - ASSAY FOR NON-FAMILIAL ALZHEIMER'S DISEASE.
Title (en)
ASSAY FOR NON-FAMILIAL ALZHEIMER'S DISEASE.
Title (de)
TEST FÜR NICHTFAMILIÄRE ALZHEIMERSCHE KRANKHEIT.
Title (fr)
DEPISTAGE DE LA MALADIE D'ALZHEIMER NON FAMILIALE.
Publication
Application
Priority
- US 9305881 W 19930618
- US 91116192 A 19920709
Abstract (en)
[origin: WO9401579A1] A method of assaying for non-familial Alzheimer's disease in a live patient, by preparing washed platelets from the blood of the patient; inserting a pH probe in the platelets in the form of a fluorescent dye such as 2',7'-bis (carboxyethyl)- 5(6)-carboxyfluorescein; inhibiting alkalinization of platelet response by blocking Na+/H+ transfer across the platelet membranes with dimethyl amiloride; stimulating the platelets with (alpha)-thrombin so that the cytoplasm of the stimulated platelets shows a characteristic acidification response or change in cytoplasmic pH; measuring the cytoplasmic pH of the platelets following stimulation; and comparing the measured pH with the corresponding stimulated cytoplasmic pH in platelets from normal individuals.
IPC 1-7
IPC 8 full level
G01N 33/50 (2006.01); G01N 33/68 (2006.01)
CPC (source: EP)
G01N 33/5091 (2013.01); G01N 33/6896 (2013.01); G01N 2800/2821 (2013.01)
Citation (search report)
- [AD] T.A. DAVIES ET AL.: "Simultaneous flow cytometric measurements of thrombin-induced cytosolic pH and Ca-ion fluxes in human platelets", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 265, no. 20, - 1 July 1990 (1990-07-01), WASHINGTON DC USA, pages 11522 - 11526, XP000561928
- [T] T.A. DAVIES ET AL.: "NON-AGE RELATED DIFFERENCES IN THROMBIN RESPONSES BY PLATELETS FROM MALE PATIENTS WITH ADVANCED ALZHEIMER'S DISEASE.", MOLECULAR BIOLOGY OF THE CELL, vol. 4, no. SUP, - 11 December 1993 (1993-12-11), NEW ORLEANS LA USA, pages 124A
- See references of WO 9401579A1
Designated contracting state (EPC)
DE FR GB
DOCDB simple family (publication)
WO 9401579 A1 19940120; EP 0649474 A1 19950426; EP 0649474 A4 19960403
DOCDB simple family (application)
US 9305881 W 19930618; EP 93916630 A 19930618