Global Patent Index - EP 0649904 B1

EP 0649904 B1 20030122 - Diagnostic reagents for the detection of antibodies to EBV

Title (en)

Diagnostic reagents for the detection of antibodies to EBV

Title (de)

Diagnostische Reagenzien zum Nachweis von Antikörper gegen EBV

Title (fr)

Réactifs diagnostiques pour la détection des anticorps contre EBV

Publication

EP 0649904 B1 20030122 (EN)

Application

EP 94202598 A 19940909

Priority

  • EP 94202598 A 19940909
  • EP 93202659 A 19930914

Abstract (en)

[origin: EP0649904A1] The present invention is directed to diagnostic reagents for the detection of antibodies against the Epstein Barr Virus and a method for the detection of antibodies to the Epstein Barr Virus in a sample. The diagnostic reagents according to the invention comprise a combination of at least part of an Epstein Barr viral structural protein, preferably a Epstein Barr VCA-protein or an Epstein Barr MA protein, and at least part of an Epstein Barr EBNA protein. Preferably, the VCA-protein is VCA-p18 protein, the MA-protein is MA-gp350/220 protein and the EBNA-protein is EBNA-1 protein. It has been found that the combination of a VCA-protein or a MA-protein, and an EBNA protein, into a single diagnostic assay yields an EBV-antibody detection method with greater sensitivity and accuracy than current methods.

IPC 1-7

C12N 15/38; G01N 33/48; C07K 14/05

IPC 8 full level

G01N 33/53 (2006.01); C07K 14/05 (2006.01); C12N 15/00 (2006.01); C12N 15/09 (2006.01); C12N 15/38 (2006.01); C12P 21/02 (2006.01); G01N 33/531 (2006.01); G01N 33/569 (2006.01); C12R 1/91 (2006.01); C12R 1/92 (2006.01)

CPC (source: EP KR US)

C07K 14/00 (2013.01 - KR); C07K 14/005 (2013.01 - EP US); G01N 33/533 (2013.01 - KR); G01N 33/56983 (2013.01 - EP US); C12N 2710/16222 (2013.01 - EP US); Y10S 436/811 (2013.01 - EP US)

Citation (examination)

  • FAERBER I. ET AL.: "Serological diagnosis of infectious mononucleosis using three anti-Epstein-Barr virus recombinant ELISAs", JOURNAL OF VIROLOGICAL METHODS, vol. 42, pages 301 - 308
  • LUKA J. ET AL.: "A sensitive enzyme-linked immunosorbent assay (ELISA) against major EBV-associated antigens. I. Correlation between ELISA and immunofluorescence titers using purified antigens.", J. IMMUNOL. METHODS, vol. 67, 1984, pages 145 - 156, XP023974557, DOI: doi:10.1016/0022-1759(84)90093-0

Designated contracting state (EPC)

AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

EP 0649904 A1 19950426; EP 0649904 B1 20030122; AT E231556 T1 20030215; AU 679545 B2 19970703; AU 7295694 A 19950330; CA 2131874 A1 19950315; CA 2131874 C 20060328; DE 69432043 D1 20030227; DE 69432043 T2 20031009; DK 0649904 T3 20030422; ES 2191673 T3 20030916; FI 113298 B 20040331; FI 944225 A0 19940913; FI 944225 A 19950315; HK 1001222 A1 19980605; JP 3547493 B2 20040728; JP H07209302 A 19950811; KR 100378942 B1 20030522; KR 950008537 A 19950419; US 5827646 A 19981027; ZA 947061 B 19950427

DOCDB simple family (application)

EP 94202598 A 19940909; AT 94202598 T 19940909; AU 7295694 A 19940913; CA 2131874 A 19940912; DE 69432043 T 19940909; DK 94202598 T 19940909; ES 94202598 T 19940909; FI 944225 A 19940913; HK 98100043 A 19980105; JP 22048894 A 19940914; KR 19940022989 A 19940913; US 30607894 A 19940914; ZA 947061 A 19940913