EP 0651795 A4 19930511 - METHOD FOR ISOLATING MUTANT CELLS.
Title (en)
METHOD FOR ISOLATING MUTANT CELLS.
Title (de)
ISOLIERUNGSVERFAHREN FÜR MUTANTE ZELLEN.
Title (fr)
PROCEDE D'ISOLEMENT DES CELLULES MUTANTES.
Publication
Application
Priority
- US 9104468 W 19910620
- US 54189590 A 19900621
Abstract (en)
[origin: WO9119799A1] A method for isolating a mutant cell that secretes a desired compound at a level greater than the starting cell from which the mutant cell is derived. The method includes providing a starting cell and a feeder cell. The starting cell has at least three characteristics. First, the starting cell has a requirement for a metabolite for survival or division of the starting cell in a solid growth medium. Second, the starting cell mutates to lack that requirement at a frequency of less than 10<-10> per cell division. Third, the starting cell has a rate of mutation of at least 10<-6> per nucleotide base per cell division. The feeder cell has at least two properties. First, the feeder cell requires the desired compound for survival or division of the feeder cell in a growth medium. Second, the feeder cell secretes the metabolite required by the starting cell into the solid growth medium. The method further includes contacting a plurality of the feeder cells and the starting cells together in a solid growth medium to allow the feeder cells to produce the desired compound and the starter cells to produce the metabolite. Both the cells will grow and divide to produce a plurality of colonies. Those colonies which are largest include one or more mutant cells.
IPC 1-7
IPC 8 full level
C12N 1/00 (2006.01); C07K 14/01 (2006.01); C07K 14/475 (2006.01); C07K 14/505 (2006.01); C07K 16/10 (2006.01); C12N 1/20 (2006.01); C12N 5/00 (2006.01); C12N 9/64 (2006.01); C12N 15/00 (2006.01); C12N 15/64 (2006.01); C12P 13/18 (2006.01); C12P 17/18 (2006.01); C12R 1/19 (2006.01)
CPC (source: EP)
C07K 14/475 (2013.01); C07K 14/505 (2013.01); C07K 16/109 (2013.01); C12N 9/6475 (2013.01); C12N 15/00 (2013.01); C12N 15/64 (2013.01); C12P 17/186 (2013.01); C07K 2319/00 (2013.01)
Citation (search report)
See references of WO 9119799A1
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IT LI LU NL SE
DOCDB simple family (publication)
WO 9119799 A1 19911226; CA 2085893 A1 19911222; EP 0651795 A1 19950510; EP 0651795 A4 19930511; JP H06501149 A 19940210
DOCDB simple family (application)
US 9104468 W 19910620; CA 2085893 A 19910620; EP 91913873 A 19910620; JP 51290491 A 19910620