Global Patent Index - EP 0652958 A1

EP 0652958 A1 19950517 - CLONING AND EXPRESSION OF A LIPASE MODULATOR GENE FROM PSEUDOMONAS PSEUDOALCALIGENES.

Title (en)

CLONING AND EXPRESSION OF A LIPASE MODULATOR GENE FROM PSEUDOMONAS PSEUDOALCALIGENES.

Title (de)

KLONIERUNG UND EXPRESSION DES LIPASE-MODULATORGENS VON PSEUDOMONAS PSEUDOALCALIGENE.

Title (fr)

CLONAGE ET EXPRESSION D'UN GENE MODULATEUR DE LIPASE A PARTIR DE GENES PSEUDOALCALINS DE PSEUDOMONAS.

Publication

EP 0652958 A1 19950517 (EN)

Application

EP 93917663 A 19930723

Priority

  • EP 93917663 A 19930723
  • EP 9301995 W 19930723
  • EP 92202281 A 19920723

Abstract (en)

[origin: WO9402617A2] The present invention discloses the cloning and expression of a lipase modulator gene obtained from a class I (Pseudomonas) species. The expression product of the modulator gene is found to give rise to a considerable increase in lipase production especially upon homologous expression. The present invention provides a method for isolating a class I lipase modulator gene, an isolated modulator gene and a class I (Pseudomonas) transformed with such a gene. Finally the present invention discloses a derivative of plasmid pJRD215 which is segregationally stable in (Pseudomonas).

IPC 1-7

C12N 15/55; C12N 9/20; C12N 1/21; C12N 15/78

IPC 8 full level

C12N 15/09 (2006.01); C07K 14/21 (2006.01); C12N 1/21 (2006.01); C12N 9/20 (2006.01); C12N 15/55 (2006.01); C12N 15/67 (2006.01); C12N 15/78 (2006.01)

CPC (source: EP)

C07K 14/21 (2013.01); C12N 9/20 (2013.01); C12N 15/67 (2013.01); C12N 15/78 (2013.01)

Citation (search report)

  • [A] J. HERMANS ET AL.: "Transformation of Mycobacterium aurum and Mycobacterium smegmatis with the broad host-range Gram-negative cosmid vector pJRD215", MOLECULAR MICROBIOLOGY, vol. 5, no. 6, 1991, pages 1561 - 1566, XP001002547
  • [DA] JOHN DAVISON ET AL.: "Vectors with restriction site banks V. pJRD215, a wide-host-range cosmid vector with multiple cloning sites", GENE, vol. 51, 1987, AMSTERDAM NL, pages 275 - 280, XP002168737
  • [A] P.K.R. KUMAR ET AL.: "Strategies for improving plasmid modofied stability in genetically modified bacteria in bioreactors", TRENDS IN BIOTECHNOLOGY, vol. 9, no. 8, 1991, pages 279 - 284, XP000233077
  • See references of WO 9402617A2

Designated contracting state (EPC)

AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 9402617 A2 19940203; WO 9402617 A3 19940609; EP 0652958 A1 19950517; JP H07509129 A 19951012

DOCDB simple family (application)

EP 9301995 W 19930723; EP 93917663 A 19930723; JP 50417694 A 19930723