Global Patent Index - EP 0653633 B1

EP 0653633 B1 20010328 - Toxicity testing assay

Title (en)

Toxicity testing assay

Title (de)

Test zum Nachweis von Toxizität

Title (fr)

Essai pour tester la toxicité

Publication

EP 0653633 B1 20010328 (EN)

Application

EP 94308382 A 19941114

Priority

US 15341593 A 19931115

Abstract (en)

[origin: EP0653633A2] A toxicity testing assay wherein a test sample is prepared with electrochromic dye and a living organism of the cladoceran order or other test organisms, and the sample is irradiated with alternately blue and yellow light to excite fluorescence. The successive groups of fluorescent emissions are then viewed at 90 DEG by a photomultiplier which develops equivalent alternate count outputs, and the count output is amplified and processed to develop data indicating (1) membrane potential of cells of the living organisms and (2) the total dye fluorescence which provides indication of deleterious effects to the organisms. <IMAGE>

IPC 1-7

G01N 33/18; G01N 21/64

IPC 8 full level

G01N 33/18 (2006.01)

CPC (source: EP)

G01N 33/186 (2013.01); Y10S 435/808 (2013.01)

Designated contracting state (EPC)

AT BE CH DE DK ES FR GB IT LI NL SE

DOCDB simple family (publication)

EP 0653633 A2 19950517; EP 0653633 A3 19960925; EP 0653633 B1 20010328; AT 200149 T 20010415; CA 2134636 A1 19950516; CA 2134636 C 20010424; DE 69426965 D1 20010503; DE 69426965 T2 20010705; DK 0653633 T3 20010507; EP 1035412 A2 20000913; ES 2155084 T3 20010501; US 5416005 A 19950516; US 5459070 A 19951017

DOCDB simple family (application)

EP 94308382 A 19941114; AT 94308382 T 19941114; CA 2134636 A 19941028; DE 69426965 T 19941114; DK 94308382 T 19941114; EP 00111066 A 19941114; ES 94308382 T 19941114; US 15341593 A 19931115; US 30683494 A 19940915