Global Patent Index - EP 0661979 A4

EP 0661979 A4 19950913 - CROSS-LINKING OLIGONUCLEOTIDES FOR ENZYME-MEDIATED TRIPLE STRAND FORMATION.

Title (en)

CROSS-LINKING OLIGONUCLEOTIDES FOR ENZYME-MEDIATED TRIPLE STRAND FORMATION.

Title (de)

VERNETZEN VON OLIGONUKLEOTIDEN FÜR ENZYMVERMITTELTE DREIFACHSTRANGBILDUNG.

Title (fr)

OLIGONUCLEOTIDES A RETICULATION POUR LA FORMATION DE TRIPLE BRIN A MEDIATION ENZYMATIQUE.

Publication

EP 0661979 A4 19950913 (EN)

Application

EP 92918930 A 19920819

Priority

  • US 9207101 W 19920819
  • US 74813891 A 19910821

Abstract (en)

[origin: WO9303736A1] The invention disclosed is directed to cross-linking between specific sites on adjoining oligonucleotides or oligodeoxynucleotides wherein the nucleoside monomers used to effect the cross-linking are (alkylating substituent substituted) pyrazolol[3,4-d]pyrimidine ribosides or 2'-deoxyribosides. This cross-linking is assisted by the presence in mammalian host of a recombination enzyme such as RecA. The cross-linking of either double or triple stranded nucleic acids is expected to have utility in the inhibiting the expression of the targeted nucleic acids in vivo and as a diagnostic tool as well.

IPC 1-7

A61K 31/70; C07H 21/02; C07H 21/04

IPC 8 full level

A61K 31/70 (2006.01); A61K 48/00 (2006.01); C07D 487/04 (2006.01); C07H 19/04 (2006.01); C07H 21/00 (2006.01); C07H 21/04 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01)

CPC (source: EP)

C07H 19/04 (2013.01); C07H 21/00 (2013.01); C12Q 1/6839 (2013.01)

C-Set (source: EP)

C12Q 1/6839 + C12Q 2525/101 + C12Q 2523/101 + C12Q 2521/507

Citation (search report)

  • [X] WO 9014353 A1 19901129 - MICROPROBE CORP [US]
  • [X] WO 8502628 A1 19850620 - HYDROCARBON RESEARCH INC [US]
  • [X] EP 0266099 A2 19880504 - UNIV JOHNS HOPKINS [US]
  • [Y] EP 0267996 A1 19880525 - TAMIR BIOTECHNOLOGY LTD [IL]
  • [X] KNORRE, D. G. ET AL: "Complementarily addressed modification of double-stranded DNA in a triple-stranded complex (English translation of the title)", DOKL. AKAD. NAUK SSSR, 1988, VOL. 300, NO. 4, PAGES 1006-9 (BIOCHEM.)
  • [PX] PETRIE CR ET AL: "A novel biotinylated adenylate analogue derived from pyrazolo[3,4-d]pyrimidine for labeling DNA probes.", BIOCONJUG CHEM, NOV-DEC 1991, VOL. 2, NO. 6, PAGES 441-6, US
  • [Y] SIDWELL R W ET AL: "In vivo antiviral properties of biologically active compounds. II. Studies with influenza and vaccinia viruses", APPL. MICROBIOL., 1968, VOL.16, NO. 2, PAGES 370-92
  • [Y] SEELA F ET AL: "Poly(adenylic acids) containing the antibiotic tubercidin -- base pairing and hydrolysis by nuclease S1.", NUCLEIC ACIDS RES, 25-2-1982, VOL. 10, NO. 4, PAGES 1389-97, GB
  • [Y] ELSNER H ET AL: "Photochemical crosslinking of protein and DNA in chromatin. II. Synthesis and application of psoralen-cystamine-arylazido photocrosslinking reagents.", ANAL BIOCHEM, SEP 1985, VOL. 149, NO. 2, PAGES 575-81, US
  • [A] TURCHINSKII, M. F. ET AL: "Conversion of noncovalent interactions in nucleoproteins into covalent bonds. III. Bisulfite-induced formation of polynucleotide-protein crosslinks in MS2 bacteriophage virions", FEBS (FED. EUR. BIOCHEM. SOC.) LETT., 1974, VOL. 38, NO. 3, PAGES 304-7
  • [A] SONENBERG N. ET AL: "Reovirus mRNA can be covalently crosslinked via the 5' cap to proteins in initiation complexes", PROC. NATL. ACAD. SCI. U. S. A., vol. 74, no. 10, US, pages 4288 - 4292
  • See references of WO 9303736A1

Designated contracting state (EPC)

AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL SE

DOCDB simple family (publication)

WO 9303736 A1 19930304; EP 0661979 A1 19950712; EP 0661979 A4 19950913; JP H06509945 A 19941110

DOCDB simple family (application)

US 9207101 W 19920819; EP 92918930 A 19920819; JP 50462393 A 19920819