Global Patent Index - EP 0663445 A1

EP 0663445 A1 19950719 - Process for producing 7beta-(4-carboxybutanamido)-cephalosporanic acid acylase ("GA) enzyme.

Title (en)

Process for producing 7beta-(4-carboxybutanamido)-cephalosporanic acid acylase ("GA) enzyme.

Title (de)

Herstellungsverfahren des Enzyms 7beta-(4-carboxy-butanamido)-cephalosporanischen Säureacylase.

Title (fr)

Procédé de production de l'acylase de l'acide 7bêta-(4-carboxy-butanamido)-cephalosporanique ("GA").

Publication

EP 0663445 A1 19950719 (EN)

Application

EP 95200057 A 19950111

Priority

IT MI940031 A 19940114

Abstract (en)

The present invention relates to a process for producing large amounts of 7 beta -(4-carboxybutanamido)-cephalosporanic acid acylase ("GA") enzyme, which process consists in growing, under suitable culture conditions, a recombinant Escherichia coli K12 strain and subsequently extracting the enzyme from the resulting culture. Enzyme production is controlled by a particular expression system which can be induced by corn-steep liquor, an extremely low cost component of the culture medium. The enzyme can be used in the enzymatic preparation of 7-amino-cephalosporanic acid by starting from 7 beta -(4-carboxybutanamido)-cephalosporanic acid. <IMAGE>

IPC 1-7

C12N 15/71; C12N 15/72; C12N 15/31; C12N 1/21; C12N 15/63; C12N 1/20

IPC 8 full level

C12N 1/21 (2006.01); C12N 9/80 (2006.01); C12N 9/86 (2006.01); C12N 15/00 (2006.01); C12N 15/31 (2006.01); C12N 15/55 (2006.01); C12N 15/70 (2006.01); C12N 15/09 (2006.01); C12P 35/02 (2006.01); C12R 1/19 (2006.01); C12R 1/38 (2006.01)

CPC (source: EP US)

C12N 9/80 (2013.01 - EP US); C12N 15/70 (2013.01 - EP US); C12P 35/02 (2013.01 - EP US)

Citation (search report)

  • [X] EP 0469919 A2 19920205 - ANTIBIOTICOS SA [ES]
  • [X] EP 0504798 A1 19920923 - HOECHST AG [DE]
  • [X] S. ICHIKAWA ET AL.: "Purification and properties of cephalosporanic acid acylase produced by mutants derived from Pseudomonas", AGRIC. BIOL. CHEM, vol. 45, no. 10, pages 2231 - 2236
  • [X] A. MATSUDA ET AL.: "Molecular cloning and structure of the gene for cephalopsoranic acid acylase from a Pseudomonas strain", J. BACTERIOL., vol. 163, no. 3, pages 1222 - 1228
  • [X] A. MATSUDA ET AL.: "Cloning and characterisation of the4 genes for two distinct cephalosporin acylases from a Pseudomonas strain", J. BACTERIOL., vol. 169, no. 12, pages 5815 - 5820
  • [X] I. ARAMORI ET AL.: "Cloning and nucleotide sequence of a novel cephalosporanic acid acylase gene of B. laterosporus and its expression in E. coli and B. subtilis", J. BACTERIOL., vol. 173, no. 24, pages 7848 - 7855
  • [X] M. ISHIYE ET AL.: "Nucleotide sequence and expression in E. coli of the cephalosporin acylase gene of a Pseudomonas strain", BIOCHEMICA BIPHYSICA ACTA, vol. 1132, pages 233 - 239, XP023469038, DOI: doi:10.1016/0167-4781(92)90155-S
  • [A] Y. SHIBUYA ET AL.: "Isolation and properties of cephalosporanic acid acylase-producing bacteria", AGRIC. BIOL. CHEM., vol. 45, no. 7, pages 1561 - 1567
  • [A] M. SRINAVASAN ET AL.: "Effect of carbon and nitrogen on exoprotease synthesis in batch cultures of B. licheniformis NCIM 2042", INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY, vol. 26, pages 22 - 24

Designated contracting state (EPC)

AT BE CH DE DK ES FR GB IE LI LU NL PT SE

DOCDB simple family (publication)

EP 0663445 A1 19950719; EP 0663445 B1 19990506; AT E179755 T1 19990515; DE 69509417 D1 19990610; DE 69509417 T2 19991216; DK 0663445 T3 19991101; ES 2132511 T3 19990816; IT 1269180 B 19970321; IT MI940031 A0 19940114; IT MI940031 A1 19950714; JP 2805594 B2 19980930; JP H07303483 A 19951121; US 5612210 A 19970318; US 5801048 A 19980901

DOCDB simple family (application)

EP 95200057 A 19950111; AT 95200057 T 19950111; DE 69509417 T 19950111; DK 95200057 T 19950111; ES 95200057 T 19950111; IT MI940031 A 19940114; JP 1986795 A 19950113; US 36645794 A 19941230; US 71238996 A 19960911