EP 0669928 A1 19950906 - APPLICATIONS OF FLUORESCENT N-NUCLEOSIDES AND FLUORESCENT STRUCTURAL ANALOGS OF N-NUCLEOSIDES.
Title (en)
APPLICATIONS OF FLUORESCENT N-NUCLEOSIDES AND FLUORESCENT STRUCTURAL ANALOGS OF N-NUCLEOSIDES.
Title (de)
ANWENDUNG VON FLUORESZIERENDEN N-NUKLEOSIDEN UND FLUORESZIERENDEN STRUKTURANALOGEN VON N-NUKLEOSIDEN.
Title (fr)
APPLICATIONS DE N-NUCLEOSIDES FLUORESCENTS ET D'ANALOGUES STRUCTURELS FLUORESCENT DE N-NUCLEOSIDES.
Publication
Application
Priority
- US 9409316 W 19940818
- US 10845793 A 19930818
Abstract (en)
[origin: WO9505391A1] Structural analogs of the six non-fluorescent N-nucleosides commonly found in RNA and DNA, which are inherently fluorescent under physiological conditions, are identified and methods for their preparation provided. Such analogs may be incorporated into DNA and/or RNA oligonucleotides via either enzymatic or chemical synthesis to produce fluorescent oligonucleotides having prescribed sequences. Such analogous sequences may be identical to, or the analogous complement of, template or target DNA or RNA sequences to which the fluorescent oligonucleotides can be hybridized. Methods of preparing either RNA or DNA oligonucleotide probes of the invention, intermediates used in such methods, and methods of using the probes of the invention in oligonucleotide amplification, detection, identification, and/or hybridization assays are also provided.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C07H 19/04 (2006.01); C07H 19/06 (2006.01); C07H 19/16 (2006.01); C07H 21/00 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP)
C07H 19/173 (2013.01); C07H 21/04 (2013.01)
Citation (search report)
See references of WO 9505391A1
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9505391 A1 19950223; CA 2145750 A1 19950223; EP 0669928 A1 19950906; JP H09505556 A 19970603
DOCDB simple family (application)
US 9409316 W 19940818; CA 2145750 A 19940818; EP 94927183 A 19940818; JP 50717495 A 19940818