EP 0678195 A1 19951025 - METHOD OF USING PRESERVED CONTROL CELLS IN THE CALIBRATION OF FLUORESCENT AND LIGHT SCATTER MEASUREMENTS.
Title (en)
METHOD OF USING PRESERVED CONTROL CELLS IN THE CALIBRATION OF FLUORESCENT AND LIGHT SCATTER MEASUREMENTS.
Title (de)
VERFAHREN ZUR KALIBRIERUNG VON FLUORESZENZ UND LICHTSTREKMESSUNGEN UNTER VERWENDUNG KONSERVIERTER KONTROLLZELLEN.
Title (fr)
PROCEDE D'UTILISATION DE CELLULES TEMOINS DANS L'ETALONNAGE DE MESURES DE L'INTENSITE FLUORESCENTE ET DE LA DISPERSION DE LUMIERE.
Publication
Application
Priority
- US 9400295 W 19940107
- US 183493 A 19930108
Abstract (en)
[origin: WO9416314A1] The invention describes a method of using differently labelled, reconstituted preserved cells as control cells in multiple color assays. These cells are used to calibrate light scatter and fluorescent intensity measurements. The use of said control cells overcomes the preservation and calibration difficulties experienced in the conventional use of fresh cells and polymer beads as controls in determining the compensation adjustments required in multiple color flow cytometry assays as a result of the overlapping wavelength regions encountered.
IPC 1-7
IPC 8 full level
G01N 33/483 (2006.01); C12P 21/08 (2006.01); C12Q 1/04 (2006.01); G01N 15/10 (2006.01); G01N 33/50 (2006.01); G01N 33/533 (2006.01); G01N 33/536 (2006.01); G01N 33/543 (2006.01); G01N 15/14 (2006.01)
CPC (source: EP)
G01N 15/1012 (2013.01); G01N 33/5005 (2013.01); G01N 33/533 (2013.01); G01N 15/14 (2013.01)
Designated contracting state (EPC)
DE ES FR GB
DOCDB simple family (publication)
WO 9416314 A1 19940721; AU 6024294 A 19940815; CA 2153344 A1 19940721; EP 0678195 A1 19951025; EP 0678195 A4 19980708; JP H08510051 A 19961022
DOCDB simple family (application)
US 9400295 W 19940107; AU 6024294 A 19940107; CA 2153344 A 19940107; EP 94906567 A 19940107; JP 51625494 A 19940107