EP 0681612 A1 19951115 - DETECTION OF PARAFFINOPHILIC MICROORGANISMS.
Title (en)
DETECTION OF PARAFFINOPHILIC MICROORGANISMS.
Title (de)
NACHWEIS PARAFFINOPHILER MIKROORGANISMEN.
Title (fr)
DETECTION DE MICROORGANISMES PARAFFINOPHILES.
Publication
Application
Priority
- US 1147993 A 19930126
- US 3535893 A 19930322
- US 9400831 W 19940121
Abstract (en)
[origin: WO9417200A1] A method of determining the presence of a paraffinophilic organism in a body specimen involves introducing portions of the body specimen into a plurality of receptacles (50-57) which contain a sterile broth and antibiotics. Subsequently, one paraffin coated slide (18) is introduced into each receptacle. After observing organism growth on the paraffin coated slides, at least one slide is subjected to an alcohol-acid fastness test to determine whether the organism is an alcohol-acid fast, an acid-fast organism or a non-acid-fast/non-alcohol-acid fast organism. If it is determined that an alcohol-acid fast organism is present on the first slide, a tellurite reduction assay is performed on a second slide to determine the possibility of a presence of paraffinophilic organism on the second slide. If the tellurite reduction assay results in a determination that there is a possibility of presence of a paraffinophilic organism on the second slide, at least one speciation assay on the third paraffin coated slide is performed to confirm the presence of a paraffinophilic organism on the third paraffin coated slide. Subsequently, DNA extraction is employed on at least one additional slide to determine whether a paraffinophilic organism is present in the body specimen.
IPC 1-7
IPC 8 full level
G01N 33/50 (2006.01); C12N 1/20 (2006.01); C12Q 1/04 (2006.01); C12Q 1/68 (2006.01)
CPC (source: EP)
C12Q 1/04 (2013.01)
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9417200 A1 19940804; AU 6127094 A 19940815; AU 671196 B2 19960815; BR 9405671 A 19951114; CA 2153901 A1 19940804; EP 0681612 A1 19951115; EP 0681612 A4 19970319; JP H08506483 A 19960716
DOCDB simple family (application)
US 9400831 W 19940121; AU 6127094 A 19940121; BR 9405671 A 19940121; CA 2153901 A 19940121; EP 94907869 A 19940121; JP 51724894 A 19940121