Global Patent Index - EP 0685071 A1

EP 0685071 A1 19951206 - METHOD FOR ABSOLUTE COUNTING OF RARE CELLS.

Title (en)

METHOD FOR ABSOLUTE COUNTING OF RARE CELLS.

Title (de)

VERFAHREN ZU ABSOLUTER ZÄHLUNG VON SELTENEN ZELLEN.

Title (fr)

PROCEDE DE COMPTAGE ABSOLU DE CELLULES RARES.

Publication

EP 0685071 A1 19951206 (EN)

Application

EP 95901205 A 19941110

Priority

  • US 9412952 W 19941110
  • US 15108693 A 19931112

Abstract (en)

[origin: WO9513540A1] A method for absolute counting of rare cells (i.e., < 5 %) in a sample of cells is described. The method utilizes flow cytometry and a reagent composition comprising fluorescent beads, one or more fluorescent markers that differentially react with cell populations in the sample and one or more fluorescent markers that selectively react with the rare cells. The method has particular utility in counting progenitor cells.

IPC 1-7

G01N 33/545; G01N 33/546; G01N 33/566

IPC 8 full level

G01N 33/49 (2006.01); G01N 15/14 (2006.01); G01N 33/50 (2006.01); G01N 33/53 (2006.01); G01N 33/542 (2006.01); G01N 33/569 (2006.01); G01N 33/577 (2006.01)

CPC (source: EP)

G01N 33/5094 (2013.01); G01N 33/56972 (2013.01)

Designated contracting state (EPC)

DE ES FR GB IT NL

DOCDB simple family (publication)

WO 9513540 A1 19950518; EP 0685071 A1 19951206; EP 0685071 A4 20001213; JP 2680931 B2 19971119; JP H08501396 A 19960213

DOCDB simple family (application)

US 9412952 W 19941110; EP 95901205 A 19941110; JP 51399895 A 19941110