EP 0685560 B1 20080402 - Method for controlling gene expression
Title (en)
Method for controlling gene expression
Title (de)
Methode zur Kontrolle genetischer Expressionen
Title (fr)
Méthode pour contrôler l'expression génétique
Publication
Application
Priority
- JP 14073694 A 19940601
- JP 20031194 A 19940803
Abstract (en)
[origin: EP0685560A2] In a lactose repressor protein, at least one amino acid at the position of 94, 241, 265 or 300 in the wild lactose repressor is replaced with an amino acid other than that of the wild lactose repressor, and the use thereof. The present mutant lactose repressor represses the expression of a desired gene at 30 DEG C or lower temperature, and induces the expression of a desired gene at 37 DEG C or higher temperature, and therefor can control the expression by change of a culture temperature without using an expensive inducer such as IPTG.
IPC 8 full level
C12N 15/09 (2006.01); C12N 15/72 (2006.01); C07K 14/00 (2006.01); C07K 14/245 (2006.01); C07K 14/575 (2006.01); C07K 14/58 (2006.01); C07K 14/585 (2006.01); C07K 14/61 (2006.01); C07K 14/62 (2006.01); C12N 15/31 (2006.01); C12P 21/00 (2006.01); C12R 1/19 (2006.01)
CPC (source: EP KR US)
C07K 14/245 (2013.01 - EP US); C12N 15/52 (2013.01 - KR); C12N 15/72 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE
Designated extension state (EPC)
LT SI
DOCDB simple family (publication)
EP 0685560 A2 19951206; EP 0685560 A3 19980107; EP 0685560 B1 20080402; AT E391182 T1 20080415; DE 69535739 D1 20080515; DE 69535739 T2 20090416; DK 0685560 T3 20080714; HU 217580 B 20000228; HU 9501578 D0 19950728; HU T72398 A 19960429; IL 113932 A0 19950831; IL 113932 A 20051120; JP 3723240 B2 20051207; JP H0848697 A 19960220; KR 100380703 B1 20030913; KR 960001124 A 19960125; US 5622840 A 19970422
DOCDB simple family (application)
EP 95303708 A 19950531; AT 95303708 T 19950531; DE 69535739 T 19950531; DK 95303708 T 19950531; HU 9501578 A 19950531; IL 11393295 A 19950531; JP 20031194 A 19940803; KR 19950014472 A 19950601; US 45692395 A 19950601