EP 0685740 A2 19951206 - Separation-free specific binding assays using anti-inhibitor antibodies.
Title (en)
Separation-free specific binding assays using anti-inhibitor antibodies.
Title (de)
Abtrennfreies, spezifisches Bindungsassay mit Anti-Inhibitor-Antikörpern.
Title (fr)
Dosage par liaison spécifique sans séparation avec des anticorps anti-inhibiteurs.
Publication
Application
Priority
US 25088394 A 19940531
Abstract (en)
Specific binding ligands can be detected with an assay which utilizes an immobilized receptor for the ligand, a reporter enzyme, an inhibitor antibody and an anti-inhibitor antibody. Both antibodies are specific for the reporter enzyme. The inhibitor antibody effectively shuts down the activity of the reporter enzyme when it is complexed thereto. The anti-inhibitor antibody binds to the reporter enzyme, does not affect the enzymatic activity, but prevents the binding of the inhibitor enzyme. This assay provides a direct correlation of the target specific binding ligand to the signal generated without the use of separation or wash steps.
IPC 1-7
IPC 8 full level
C12Q 1/00 (2006.01); C12Q 1/28 (2006.01); G01N 33/531 (2006.01); G01N 33/543 (2006.01); G01N 33/58 (2006.01)
CPC (source: EP US)
G01N 33/54306 (2013.01 - EP US); G01N 33/581 (2013.01 - EP US); Y10S 435/962 (2013.01 - EP); Y10S 435/969 (2013.01 - EP); Y10S 435/97 (2013.01 - EP); Y10S 435/975 (2013.01 - EP); Y10S 436/816 (2013.01 - EP); Y10S 436/822 (2013.01 - EP)
Citation (applicant)
- US 3935074 A 19760127 - RUBENSTEIN KENNETH EDWARD, et al
- EP 0323692 A2 19890712 - EASTMAN KODAK CO [US]
- US 5262297 A 19931116 - SUTTON RICHARD C [US], et al
- US 4997772 A 19910305 - SUTTON RICHARD C [US], et al
- US 5252457 A 19931012 - SNODGRASS GARY L [US], et al
- US 4383031 A 19830510 - BOGUSLASKI ROBERT C, et al
- US 4598044 A 19860701 - KRICKA LARRY J [GB], et al
- US 4729950 A 19880308 - KRICKA LARRY J [GB], et al
- US 5108893 A 19920428 - BARET ALAIN [FR]
- US 4087747 A 19780502 - DEEGEN AXEL W, et al
- US 5024935 A 19910618 - MCCLUNE GREGORY J [US], et al
- US 3992158 A 19761116 - PRZYBYLOWICZ EDWIN P, et al
- US 4258001 A 19810324 - PIERCE ZONA R, et al
- US 4292272 A 19810929 - KITAJIMA MASAO, et al
- US 4430436 A 19840207 - KOYAMA MIKIO [JP], et al
- EP 0517327 A2 19921209 - EASTMAN KODAK CO [US]
- US 5177023 A 19930105 - SUTTON RICHARD C [US], et al
- HENDERSON ET AL., CLIN. CHEM., vol. 32, no. 9, 1986, pages 1637 - 1641
- J. IMMUNOL. METHODS, vol. 81, 1985, pages 223 - 228
- WILLIAMS ET AL.: "Method in Immunology and Immunochemistry", 1976, ACADEMIC PRESS, NEW YORK
- YOSHITAKE ET AL., EUR. J. BIOCHEM., vol. 101, 1979, pages 395
- GUNDERMANN ET AL.: "Chemiluminescence in Organic Chemistry", 1987, SPRINGER-VERLAG, BERLIN, pages: 204 - 207
Designated contracting state (EPC)
BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
EP 0685740 A2 19951206; EP 0685740 A3 19960403; EP 0685740 B1 20020403; AU 2041095 A 19951207; CA 2150496 A1 19951201; DE 69526143 D1 20020508; DE 69526143 T2 20020926; JP H0854392 A 19960227; US 5565326 A 19961015
DOCDB simple family (application)
EP 95303655 A 19950530; AU 2041095 A 19950531; CA 2150496 A 19950530; DE 69526143 T 19950530; JP 13399695 A 19950531; US 25088394 A 19940531