EP 0687302 B1 20000503 - BI-FUNCTIONAL EXPRESSION SYSTEM
Title (en)
BI-FUNCTIONAL EXPRESSION SYSTEM
Title (de)
BIFUNKTIONELLES EXPRESSIONS SYSTEM
Title (fr)
SYSTEME D'EXPRESSION BIFONCTIONNEL
Publication
Application
Priority
- GB 9400373 W 19940225
- GB 9303988 A 19930226
Abstract (en)
[origin: US5695961A] PCT No. PCT/GB94/00373 Sec. 371 Date Sep. 8, 1995 Sec. 102(e) Date Sep. 8, 1995 PCT Filed Feb. 25, 1994 PCT Pub. No. WO94/19472 PCT Pub. Date Sep. 1, 1994A novel expression system is provided comprising a DNA sequence containing transcriptional and translational signals that promote the over production of recombinant proteins both in bacterial hosts (e.g., Escherichia coli) and yeasts (e.g., Saccharomyces cerevisiae). The design of the expression system lends itself to a unique strategy which allows heterologous genes to be directly cloned at a position relative to the transcription/translation signals which is optimal for expression. Particularly provided are expression cassettes comprising a sequence of the invention combined with a purpose built series of plasmids wherein the utility and efficiency of the resultant expression vectors can be demonstrated to over produce protein, particularly phenylalanine ammonia lyase (herein abbreviated to PAL), in E. coli and S. cerevisiae to levels hitherto unattainable.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 9/88 (2006.01); C12N 15/66 (2006.01); C12N 15/70 (2006.01); C12N 15/81 (2006.01); C12P 21/02 (2006.01); C12R 1/19 (2006.01); C12R 1/21 (2006.01)
CPC (source: EP US)
C12N 9/88 (2013.01 - EP US); C12N 15/66 (2013.01 - EP US); C12N 15/70 (2013.01 - EP US); C12N 15/81 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE CH DE DK FR GB IT LI NL SE
DOCDB simple family (publication)
WO 9419472 A2 19940901; WO 9419472 A3 19941124; AT E192497 T1 20000515; AU 6042194 A 19940914; AU 678813 B2 19970612; CA 2156260 A1 19940901; DE 69424278 D1 20000608; EP 0687302 A1 19951220; EP 0687302 B1 20000503; GB 2297327 A 19960731; GB 2297327 B 19970416; GB 9303988 D0 19930414; GB 9516364 D0 19951011; JP H09503122 A 19970331; US 5695961 A 19971209
DOCDB simple family (application)
GB 9400373 W 19940225; AT 94906977 T 19940225; AU 6042194 A 19940225; CA 2156260 A 19940225; DE 69424278 T 19940225; EP 94906977 A 19940225; GB 9303988 A 19930226; GB 9516364 A 19940225; JP 51877394 A 19940225; US 50745595 A 19950908