EP 0689610 B1 20020703 - DNA SEQUENCING BY MASS SPECTROMETRY VIA EXONUCLEASE DEGRADATION
Title (en)
DNA SEQUENCING BY MASS SPECTROMETRY VIA EXONUCLEASE DEGRADATION
Title (de)
DNS-SEQUENZBESTIMMUNG DURCH MASSENSPEKTROMETRIE AUF DEM WEG DES ABBAUS MIT EXONUKLEASE
Title (fr)
SEQUENCAGE DE L'ADN AU MOYEN DE LA SPECTROMETRIE DE MASSE PAR DEGRADATION A L'EXONUCLEASE
Publication
Application
Priority
- US 9402938 W 19940318
- US 3473893 A 19930319
Abstract (en)
[origin: US5622824A] Methods for determining the sequence of nucleic acids by cleaving the nueleic acid unilaterally from a first end with an exonuclease activity to sequentially release individual nucleotides, identifying each of the sequentially release nucleotides by mass spectrometry, and determining the sequence of the nueleic acid from the identified nucleotides are disclosed. The method is amenable to multiplexing for simultaneously determining more than one nucleic acid sequence.
IPC 1-7
IPC 8 full level
G01N 33/50 (2006.01); C07F 9/24 (2006.01); C07H 19/06 (2006.01); C07H 19/10 (2006.01); C07H 19/16 (2006.01); C07H 19/20 (2006.01); C07H 21/00 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6872 (2018.01); G01N 35/10 (2006.01)
CPC (source: EP US)
C07F 9/2408 (2013.01 - EP US); C07F 9/2429 (2013.01 - EP US); C07H 19/06 (2013.01 - EP US); C07H 19/10 (2013.01 - EP US); C07H 19/16 (2013.01 - EP US); C07H 19/20 (2013.01 - EP US); C07H 21/00 (2013.01 - EP US); C12Q 1/6872 (2013.01 - EP US); G01N 35/1067 (2013.01 - EP US)
C-Set (source: EP US)
Citation (examination)
- EP 0612994 A2 19940831 - CIBA GEIGY AG [CH]
- WO 9011369 A1 19901004 - HOLMES MICHAEL JOHN [GB], et al
- WO 9201812 A1 19920206 - CEMU BIOTEKNIK AB [SE]
- EP 0371437 A2 19900606 - ORION YHTYMAE OY [FI]
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
US 5622824 A 19970422; AT E220114 T1 20020715; AU 6411694 A 19941011; AU 687801 B2 19980305; CA 2158642 A1 19940929; DE 69430909 D1 20020808; DE 69430909 T2 20030227; EP 0689610 A1 19960103; EP 0689610 B1 20020703; JP H08507926 A 19960827; US 5851765 A 19981222; US 5872003 A 19990216; WO 9421822 A1 19940929
DOCDB simple family (application)
US 38817195 A 19950210; AT 94911643 T 19940318; AU 6411694 A 19940318; CA 2158642 A 19940318; DE 69430909 T 19940318; EP 94911643 A 19940318; JP 52125594 A 19940318; US 45349995 A 19950530; US 45452795 A 19950530; US 9402938 W 19940318