EP 0690927 A1 19960110 - FLUORESCENT DETECTION OF NONVIABLE CELLS
Title (en)
FLUORESCENT DETECTION OF NONVIABLE CELLS
Title (de)
NACHWEIS NICHT LEBENDER ZELLEN MITTELS FLUORESZENZ
Title (fr)
DETECTION PAR FLUORESCENCE DE CELLULES NON VIABLES
Publication
Application
Priority
- US 99693 A 19930106
- US 9400214 W 19940106
Abstract (en)
[origin: WO9416102A1] A method for fluorescent discrimination of cells that are nonviable prior to lysis or fixation techniques using a complementary pair of nucleic acid dyes which competitively bind to nucleic acid but which differ in their fluorescent emission characteristics. An especially preferred pair of dyes is 7-aminoactinomycin D (7-AAD) and actinomycin D (AD).
IPC 1-7
IPC 8 full level
G01N 33/566 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6816 (2018.01); C12Q 1/6841 (2018.01); G01N 21/78 (2006.01); G01N 33/50 (2006.01); G01N 33/569 (2006.01)
CPC (source: EP)
C12Q 1/6816 (2013.01); C12Q 1/6841 (2013.01); G01N 33/5094 (2013.01); G01N 33/56966 (2013.01)
Designated contracting state (EPC)
DE ES FR GB IT
DOCDB simple family (publication)
WO 9416102 A1 19940721; CA 2153257 A1 19940721; EP 0690927 A1 19960110; EP 0690927 A4 20020213; JP H08507370 A 19960806
DOCDB simple family (application)
US 9400214 W 19940106; CA 2153257 A 19940106; EP 94906050 A 19940106; JP 51621794 A 19940106