EP 0693131 A4 19980610 - CELL FREE SYSTEM FOR PROTEIN SYNTHESIS AND USE OF CHAPERONE PROTEINS THEREIN
Title (en)
CELL FREE SYSTEM FOR PROTEIN SYNTHESIS AND USE OF CHAPERONE PROTEINS THEREIN
Title (de)
ZELLFREIES SYSTEM FÜR DIE PROTEINSYNTHESE UND DIE VERWENDUNG VON CHAPERONPROTEINEN DABEI
Title (fr)
SYSTEME ACELLULAIRE S'APPLIQUANT A LA SYNTHESE PROTEIQUE ET UTILISATION DE PROTEINES CHAPERONS
Publication
Application
Priority
- US 9403860 W 19940408
- US 4544593 A 19930408
- US 21997194 A 19940404
Abstract (en)
[origin: WO9424303A1] The present invention provides a novel high efficiency method for the cell free engineering and synthesis of protein. A novel method of the present invention comprises the steps of: preparing a cell free extract; separating out a ribosome fraction from said extract; incubating said ribosome fraction in the presence of a transcription/translation medium; and measuring the amount of protein synthesized. The method of the present invention may be used as a coupled transcription/translation system, a translation only system or a cell-free continuous flow system. Also provided are methods for synthesis of proteins and their correct folding using chaperone proteins.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C12P 21/02 (2006.01)
CPC (source: EP KR)
C07K 1/14 (2013.01 - KR); C07K 1/16 (2013.01 - KR); C12P 21/02 (2013.01 - EP)
Citation (search report)
- [XY] EP 0401369 A1 19901212 - INST BELKA AKAD NAUK SSSR [SU]
- [XY] KIGAWA T ET AL: "A CONTINUOUS CELL-FREE PROTEIN SYNTHESIS SYSTEM FOR COUPLED TRANSCRIPTION-TRANSLATION", JOURNAL OF BIOCHEMISTRY, vol. 110, 1991, pages 166 - 168, XP002044224
- [Y] GETHING M.J. & SAMBROOK J.: "Protein folding in the cell", NATURE, vol. 355, - 2 January 1992 (1992-01-02), pages 33 - 45, XP002060491
- See references of WO 9424303A1
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9424303 A1 19941027; AU 6628894 A 19941108; AU 693443 B2 19980702; CA 2159899 A1 19941027; EP 0693131 A1 19960124; EP 0693131 A4 19980610; IL 109262 A0 19940826; JP H08508651 A 19960917; KR 960701892 A 19960328; NZ 265504 A 19960925
DOCDB simple family (application)
US 9403860 W 19940408; AU 6628894 A 19940408; CA 2159899 A 19940408; EP 94914083 A 19940408; IL 10926294 A 19940408; JP 52331994 A 19940408; KR 19950704388 A 19951007; NZ 26550494 A 19940408