EP 0698085 A1 19960228 - FLOW-THROUGH BIOREACTOR WITH GROOVES FOR CELL RETENTION
Title (en)
FLOW-THROUGH BIOREACTOR WITH GROOVES FOR CELL RETENTION
Title (de)
DURCHFLUSS-BIOREAKTOR MIT RILLEN ZUR ZURÜCKHALTUNG VON ZELLEN
Title (fr)
BIOREACTEUR A ECOULEMENT CONTINU DOTE DE RAINURES DE RETENTION DES CELLULES
Publication
Application
Priority
- US 9502686 W 19950303
- US 20966094 A 19940311
- US 34910294 A 19941202
Abstract (en)
[origin: WO9524464A1] The invention is a flow-through bioreactor for the retention and culture of cells in perfused media. The bioreactor is a generally rectangular vessel with inlet and outlet ports in the lid allowing for media flow along the longitudinal axis of the vessel. The inner surface of the bottom wall of the bioreactor has a plurality of generally rectangular grooves having a length, a depth, and a width. The grooves are positioned in the bottom wall such that their length is transverse to the longitudinal axis of the vessel, allowing media flow across the width of the grooves. Cells settle into the grooves, where they proliferate and differentiate, without entering the bulk flow of media through the vessel, thus avoiding loss of cells due to media flow. The invention also provides a method for the perfusion culture of hematopoietic cells whereby a suspension of either unselected hematopoietic mononuclear cells or CD34+ selected cells is placed in the bioreactor and cultured without loss of non-adherent stem/progenitor cells. Various cytokines can be added to the culture medium such that the cells in the grooves form colony-forming units (CFU-GM, BFU-E, CFU-Mix), long-term culture initiating cells (LTC-IC), and granulocytic precursors (blast cells, promyelocytes, myelocytes, metamyelocytes).
IPC 1-7
C12M 3/00; C12M 3/04; C12N 5/00; C12N 5/06; C12N 5/12; C12N 5/16
IPC 8 full level
C12M 3/00 (2006.01); C12M 3/04 (2006.01); C12N 5/07 (2010.01); C12N 5/071 (2010.01); C12N 5/0789 (2010.01)
CPC (source: EP)
C12M 23/04 (2013.01); C12M 23/12 (2013.01); C12M 25/06 (2013.01); C12M 29/10 (2013.01)
Designated contracting state (EPC)
DE FR GB IT
DOCDB simple family (publication)
WO 9524464 A1 19950914; AU 2060195 A 19950925; AU 687531 B2 19980226; CA 2162465 A1 19950914; EP 0698085 A1 19960228; EP 0698085 A4 19960821; IL 112944 A0 19950629; IL 112944 A 19970218; JP H09501324 A 19970210
DOCDB simple family (application)
US 9502686 W 19950303; AU 2060195 A 19950303; CA 2162465 A 19950303; EP 95912718 A 19950303; IL 11294495 A 19950309; JP 52354995 A 19950303