Global Patent Index - EP 0702715 A4

EP 0702715 A4 2000-01-19 - CLONING AND EXPRESSION OF THE CHONDROITINASE I AND II GENES FROM $i(P. VULGARIS)

Title (en)

CLONING AND EXPRESSION OF THE CHONDROITINASE I AND II GENES FROM $i(P. VULGARIS)

Title (de)

KLONIERUNG UND EXPRESSION DER CHONDROITINASE I- UND II-GENE AUS $i(P. VULGARIS)

Title (fr)

CLONAGE ET EXPRESSION DE GENES DE CHONDROITINASE I ET II A PARTIR DE $i(P. VULGARIS)

Publication

EP 0702715 A4 (EN)

Application

EP 94916561 A

Priority

  • US 9404495 W
  • US 5220693 A
  • US 5361593 A

Abstract (en)

[origin: WO9425567A1] This invention relates to the DNA sequence encoding the major protein component of chondroitinase ABC, which is referred to as "chondroitinase I", from Proteus vulgaris (P. vulgaris), which is contained in the Nsi fragment shown in the figure. This invention further relates to the DNA sequence encoding a second protein component of chondroitinase ABC, which is referred to as "chondroitinase II", from P. vulgaris, to the cloning and expression of the genes containing these DNA sequences, to the amino acid sequences of the recombinant chondroitinase I and II, and to methods for the isolation and purification of recombinant chondroitinase I or II. These methods provide significantly higher yields and purity than those obtained by adapting for the recombinant enzymes the method previously used for isolating and purifying native chondroitinase I enzyme from P. vulgaris.

IPC 1-7

C12N 1/21; C12N 9/88; C12N 15/31; C12N 15/60; C12N 15/61

IPC 8 full level

C12N 1/21 (2006.01); C12N 9/88 (2006.01); C12N 15/31 (2006.01); C12R 1/19 (2006.01)

CPC

C12N 9/88 (2013.01)

Citation (search report)

  • [XY] GB 1067253 A 19670503 - BIOREX LABORATORIES LTD
  • [PX] EP 0576294 A2 19931229 - SEIKAGAKU KOGYO CO LTD [JP]
  • [E] EP 0613949 A2 19940907 - MARUHA CORP [JP]
  • [XY] TAKEGAWA, K. ET AL.: "Effect of mucopolysaccharides on chondroitinase production and rapid isolation of chondroitinase", TECH. BULL. FAC. AGR. KAGAWA UNIV., vol. 45, no. 2, 1993, pages 111 - 114, XP002113501
  • [PX] DATABASE WPI Derwent World Patents Index; AN 94-155922, XP002113502, "chondroitinase and its gene ..."
  • See also references of WO 9425567A1

DCS

AT BE CH DE DK ES FR GB GR IE IT LI LU NL PT SE

DOCDB simple family

WO 9425567 A1 19941110; AU 6818394 A 19941121; AU 697156 B2 19981001; CA 2161125 A1 19941110; EP 0702715 A1 19960327; EP 0702715 A4 20000119; JP H09500011 A 19970107