EP 0707646 A1 19960424 - VECTORS FOR GENETICALLY ENGINEERED CELLS THAT PRODUCE INSULIN IN RESPONSE TO GLUCOSE
Title (en)
VECTORS FOR GENETICALLY ENGINEERED CELLS THAT PRODUCE INSULIN IN RESPONSE TO GLUCOSE
Title (de)
VEKTOREN FÜR GENTEMANIPULIERTE ZELLEN, DIE INSULIN ALS REAKTION AUF GLUKOSE PRODUZIEREN
Title (fr)
VECTEURS POUR DES CELLULES OBTENUES PAR TECHNIQUE GENETIQUE PRODUISANT DE L'INSULINE EN REACTION AU GLUCOSE
Publication
Application
Priority
- US 9407321 W 19940628
- US 8474293 A 19930628
Abstract (en)
[origin: WO9500644A1] The present disclosure relates to the application of genetic engineering to provide artificial beta cells, i.e. cells which can secrete insulin in response to glucose. This is achieved preferably through the introduction of one or more genes selected from the insulin gene, glucokinase gene, and the glucose transporter gene GLUT-2, so as to provide an engineered cell having all three of these genes in a biologically functional and responsive configuration. Advantageous methods for adenovirus-mediated gene transfer into islet cells are described, as are recombinant adenovirus constructs containing genes involved in glucose sensing, including GLUT-2, GLUT-1 and normal and disease-associated glucokinase genes. Also disclosed are methods for the detection of diabetes-associated antigens, and methods employing engineered cells in the large-scale production of human insulin.
IPC 1-7
C12N 15/12; C12N 15/54; C12N 9/12; C12N 7/01; C12N 5/10; C12N 15/86; C12P 21/02; A61K 31/71; A61K 48/00
IPC 8 full level
C07K 14/62 (2006.01); C12N 9/10 (2006.01); C12N 15/12 (2006.01); C12N 15/861 (2006.01)
CPC (source: EP)
C07K 14/62 (2013.01); C12N 9/10 (2013.01); C12N 15/86 (2013.01); C12N 2710/10343 (2013.01); C12N 2830/008 (2013.01)
Citation (search report)
See references of WO 9500644A1
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9500644 A1 19950105; AU 687836 B2 19980305; AU 7179994 A 19950117; CA 2166078 A1 19950105; EP 0707646 A1 19960424
DOCDB simple family (application)
US 9407321 W 19940628; AU 7179994 A 19940628; CA 2166078 A 19940628; EP 94920831 A 19940628