Title (en)

RECEPTOR FUNCTION ASSAYS

Title (de)

REZEPTORFUNKTIONSTESTS

Title (fr)

PROCEDES DE DETERMINATION DE LA FONCTION D'UN RECEPTEUR

Publication

EP 0711341 A4 20011219 (EN)

Application

EP 94924067 A 19940729

Priority

• US 9408571 W 19940729
• US 9985093 A 19930729

Abstract (en)

[origin: WO9504136A1] Methods are provided for assaying the function (e.g., activation) of signal-transducing receptors on cells. The methods are useful for identifying compounds that are agonists or antagonists of receptor function. The assays utilize a two-antibody sandwich assay employing an immobilized first antibody that specifically binds and captures the receptor of interest along with any tightly associated proteins or polypeptides and a second antibody directed against (i) an epitope of the receptor that is characteristic of the activated but not the unactivated state, (ii) an epitope of a protein or molecule characteristically bound to activated receptor but not to unactivated receptor, or (iii) an epitope of the receptor that is characteristic of the unactivated but not the activated state. In one embodiment, the level of binding of the second antibody is determined and correlated with the presence of activated receptor, and thus with activation in response to the treatment received by the cells. The methods provided by the invention will find particular use for detecting activation of tyrosine kinase growth factor receptors.

IPC 1-7

C12N 9/12; G01N 33/53; G01N 33/543; G01N 33/545; G01N 33/573; G01N 33/566

IPC 8 full level

G01N 33/50 (2006.01); G01N 33/543 (2006.01); G01N 33/566 (2006.01)

CPC (source: EP)

G01N 33/5008 (2013.01); G01N 2333/9121 (2013.01)

Citation (search report)

• [X] WO 9213870 A1 19920820 - UNIV CALIFORNIA [US]
• [E] EP 1000617 A2 20000517 - SUGEN INC [US], et al
• [E] WO 9514930 A1 19950601 - GENENTECH INC [US], et al
• See references of WO 9504136A1

Designated contracting state (EPC)

AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 9504136 A1 19950209; AU 7408094 A 19950228; CA 2166871 A1 19950209; EP 0711341 A1 19960515; EP 0711341 A4 20011219; JP H09501767 A 19970218; SG 63596 A1 19990330

DOCDB simple family (application)

US 9408571 W 19940729; AU 7408094 A 19940729; CA 2166871 A 19940729; EP 94924067 A 19940729; JP 50599295 A 19940729; SG 1996005770 A 19940729