Global Patent Index - EP 0711341 A4

EP 0711341 A4 2001-12-19 - RECEPTOR FUNCTION ASSAYS

Title (en)

RECEPTOR FUNCTION ASSAYS

Title (de)

REZEPTORFUNKTIONSTESTS

Title (fr)

PROCEDES DE DETERMINATION DE LA FONCTION D'UN RECEPTEUR

Publication

EP 0711341 A4 (EN)

Application

EP 94924067 A

Priority

  • US 9408571 W
  • US 9985093 A

Abstract (en)

[origin: WO9504136A1] Methods are provided for assaying the function (e.g., activation) of signal-transducing receptors on cells. The methods are useful for identifying compounds that are agonists or antagonists of receptor function. The assays utilize a two-antibody sandwich assay employing an immobilized first antibody that specifically binds and captures the receptor of interest along with any tightly associated proteins or polypeptides and a second antibody directed against (i) an epitope of the receptor that is characteristic of the activated but not the unactivated state, (ii) an epitope of a protein or molecule characteristically bound to activated receptor but not to unactivated receptor, or (iii) an epitope of the receptor that is characteristic of the unactivated but not the activated state. In one embodiment, the level of binding of the second antibody is determined and correlated with the presence of activated receptor, and thus with activation in response to the treatment received by the cells. The methods provided by the invention will find particular use for detecting activation of tyrosine kinase growth factor receptors.

IPC 1-7 (main, further and additional classification)

C12N 9/12; G01N 33/53; G01N 33/543; G01N 33/545; G01N 33/566; G01N 33/573

IPC 8 full level (invention and additional information)

G01N 33/543 (2006.01); G01N 33/50 (2006.01); G01N 33/566 (2006.01)

CPC (invention and additional information)

G01N 33/5008 (2013.01); G01N 2333/9121 (2013.01)

Citation (search report)

Designated contracting state (EPC)

AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE

EPO simple patent family

WO 9504136 A1 19950209; AU 7408094 A 19950228; CA 2166871 A1 19950209; EP 0711341 A1 19960515; EP 0711341 A4 20011219; JP H09501767 A 19970218; SG 63596 A1 19990330

INPADOC legal status


2004-08-25 [18D] APPLICATION DEEMED TO BE WITHDRAWN

- Effective date: 20040202

2001-12-19 [A4] DESPATCH OF SUPPLEMENTARY SEARCH REPORT

- Effective date: 20011102

2001-12-19 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A4

- Designated State(s): AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE

2001-12-19 [RIC1] CLASSIFICATION (CORRECTION)

- Free text: 7C 12N 9/12 A, 7G 01N 33/53 B, 7G 01N 33/543 B, 7G 01N 33/545 B, 7G 01N 33/573 B, 7G 01N 33/566 B

1996-05-15 [17P] REQUEST FOR EXAMINATION FILED

- Effective date: 19960115

1996-05-15 [AK] DESIGNATED CONTRACTING STATES:

- Kind Code of Ref Document: A1

- Designated State(s): AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE