Title (en)

PRO-UROKINASE MUTANTS

Title (de)

MUTANTEN DER PRO-UROKINASE

Title (fr)

PRO-UROKINASES OBTENUES PAR MUTATION

Publication

EP 0714435 A4 19970820 (EN)

Application

EP 94922037 A 19940628

Priority

• US 9407278 W 19940628
• US 8716393 A 19930702

Abstract (en)

[origin: WO9501427A1] The invention relates to thrombolytically active pro-urokinase (pro-UK) mutants comprising the amino acid sequence of native pro-UK, but including a mutation which causes the pro-UK mutants to induce less fibrinogenolysis and non-specific plasminogen activation than native pro-UK, to have at least a 10-fold lower intrinsic activity than native pro-UK, and to have substantially the same fibrin promotion and thrombolytic activity after plasmin activation compared to native pro-UK when administered to a patient. The figure depicts the flexible loop (amino acids 297-313), in which a substitution occurs.

IPC 1-7

C12N 9/48; C12N 9/72; C12N 15/57; C12N 15/58; A61K 38/46; A61K 38/48

IPC 8 full level

C12N 15/09 (2006.01); A61K 38/46 (2006.01); A61P 7/02 (2006.01); C07K 14/745 (2006.01); C12N 1/15 (2006.01); C12N 1/19 (2006.01); C12N 1/21 (2006.01); C12N 5/10 (2006.01); C12N 9/72 (2006.01); C12N 15/57 (2006.01); A61K 38/00 (2006.01); C12R 1/91 (2006.01)

CPC (source: EP US)

A61P 7/02 (2017.12 - EP); C12N 9/6459 (2013.01 - EP US); C12N 9/6462 (2013.01 - EP US); C12Y 304/21069 (2013.01 - EP US); C12Y 304/21073 (2013.01 - EP US); A61K 38/00 (2013.01 - EP US)

Citation (search report)

• [A] EP 0387380 A1 19900919 - BEHRINGWERKE AG [DE]
• [A] WO 8907641 A1 19890824 - GENZYME CORP [US]
• [PX] CHEMICAL ABSTRACTS, vol. 121, no. 17, 24 October 1994, Columbus, Ohio, US; abstract no. 197128, UESHIMA, S. ET AL: "Expression and characterization of clustered charge-to-alanine mutants of low Mr single-chain urokinase -type plasminogen activator" XP002032187 & THROMB. HAEMOSTASIS (1994), 71(1), 134-40 CODEN: THHADQ;ISSN: 0340-6245, 1994
• See references of WO 9501427A1

Designated contracting state (EPC)

AT BE CH DE ES FR GB LI LU NL PT

DOCDB simple family (publication)

WO 9501427 A1 19950112; AT E239084 T1 20030515; CA 2165977 A1 19950112; CN 1115412 C 20030723; CN 1130402 A 19960904; DE 69432592 D1 20030605; DE 69432592 T2 20040401; EP 0714435 A1 19960605; EP 0714435 A4 19970820; EP 0714435 B1 20030502; EP 1361276 A1 20031112; ES 2197165 T3 20040101; JP 2004337172 A 20041202; JP H08511953 A 19961217; NZ 269061 A 19970324; PT 714435 E 20030829; US 5472692 A 19951205

DOCDB simple family (application)

US 9407278 W 19940628; AT 94922037 T 19940628; CA 2165977 A 19940628; CN 94193246 A 19940628; DE 69432592 T 19940628; EP 03009709 A 19940628; EP 94922037 A 19940628; ES 94922037 T 19940628; JP 2004200871 A 20040707; JP 50359895 A 19940628; NZ 26906194 A 19940628; PT 94922037 T 19940628; US 8716393 A 19930702