EP 0726312 B1 20011212 - Methods for capture and selective release of nucleic acids
Title (en)
Methods for capture and selective release of nucleic acids
Title (de)
Verfahren zum Einfangen und selektiven Freisetzen von Nukleinsäure
Title (fr)
Procédés pour la capture et la libération sélective d'acides nucléiques
Publication
Application
Priority
US 30576994 A 19940913
Abstract (en)
[origin: EP0726312A2] Nucleic acids can be made available for amplification or other treatment after lysis by contacting the lysate with polyethyleneimine to form a precipitate with the nucleic acids. The nucleic acids are then released from the precipitate by contact with a strong base, and the released nucleic acids are kept in solution with an anionic phosphate ester surfactant. This method for preparing specimen samples is simple and quite rapid. <IMAGE>
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C07H 21/04 (2006.01); C12N 15/10 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6806 (2018.01)
CPC (source: EP US)
C12N 15/1003 (2013.01 - EP US); C12Q 1/6806 (2013.01 - EP US)
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
EP 0726312 A2 19960814; EP 0726312 A3 19980114; EP 0726312 B1 20011212; AT E210725 T1 20011215; CA 2157968 A1 19960314; CA 2157968 C 20091110; DE 69524560 D1 20020124; DE 69524560 T2 20020529; JP 3727692 B2 20051214; JP H08173159 A 19960709; US 5622822 A 19970422
DOCDB simple family (application)
EP 95306363 A 19950912; AT 95306363 T 19950912; CA 2157968 A 19950911; DE 69524560 T 19950912; JP 23379695 A 19950912; US 30576994 A 19940913