EP 0731842 A1 19960918 - RECOMBINANT BINDING PROTEINS AND PEPTIDES

Title (en)

RECOMBINANT BINDING PROTEINS AND PEPTIDES

Title (de)

REKOMBINANTE INDUNGSPROTEINE UND -PEPTIDE

Title (fr)

PROTEINES ET PEPTIDES DE LIAISON RECOMBINES

Publication

EP 0731842 A1 19960918 (EN)

Application

EP 95902235 A 19941205

Priority

GB 9402662 W 19941205
GB 9302492 W 19931203
GB 9412147 A 19940617

Abstract (en)

[origin: WO9515388A1] DNA constructs comprise a first exon sequence of nucleotides encoding a first peptide or polypeptide, a second exon sequence of nucleotides encoding a second peptide or polypeptide and a third sequence of nucleotides between the first and second sequences encoding a heterologous intron, for example that of Tetrahymena thermophila nuclear pre-rRNA, between RNA splice sites and a site-specific recombination sequence, such as loxP, within the intron, the exons together encoding a product peptide or polypeptide. Such constructs are of use in methods of production of peptides or polypeptides, transcription leading to splicing out of the intron enabling translation of a single chain product peptide or polypeptide. Isolated nucleic acid constructs consisting essentially of a sequence of nucleotides encoding a self-splicing intron with a site-specific recombination sequence within the intron, for use in creation of constructs for expression of peptides or polypeptides, are also provided.

IPC 1-7

C12N 15/62; C12N 15/13; C12N 15/12; C12N 15/70; C12N 1/21; C12N 9/00

IPC 8 full level

C12N 15/02 (2006.01); C07K 16/00 (2006.01); C12N 1/21 (2006.01); C12N 9/00 (2006.01); C12N 15/09 (2006.01); C12N 15/10 (2006.01); C12N 15/113 (2010.01); C12P 21/02 (2006.01); C12P 21/08 (2006.01); C40B 40/02 (2006.01); C12R 1/19 (2006.01)

CPC (source: EP)

C07K 16/00 (2013.01); C07K 16/40 (2013.01); C07K 16/44 (2013.01); C07K 16/468 (2013.01); C12N 15/1037 (2013.01); C12N 15/113 (2013.01); C40B 40/02 (2013.01); C07K 2319/00 (2013.01); C12N 2310/111 (2013.01); C12N 2310/1241 (2013.01); C12N 2310/127 (2013.01); C12N 2800/30 (2013.01)

Citation (search report)

See references of WO 9515388A1

Designated contracting state (EPC)

AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 9515388 A1 19950608; AU 1117095 A 19950619; AU 690171 B2 19980423; CA 2177367 A1 19950608; EP 0731842 A1 19960918; JP H09506508 A 19970630

DOCDB simple family (application)

GB 9402662 W 19941205; AU 1117095 A 19941205; CA 2177367 A 19941205; EP 95902235 A 19941205; JP 51550595 A 19941205