Global Patent Index - EP 0733122 A4

EP 0733122 A4 19980610 - FUSION PROTEINS CONTAINING ADENO-ASSOCIATED VIRUS REP PROTEIN AND BACTERIAL PROTEIN

Title (en)

FUSION PROTEINS CONTAINING ADENO-ASSOCIATED VIRUS REP PROTEIN AND BACTERIAL PROTEIN

Title (de)

FUSIONSPROTEINE, DIE DAS REP-PROTEIN DES ADENO-ASSOZIIERTEN VIRUS ENTHALTEN, UND PROTEINE AUS BAKTERIEN

Title (fr)

PROTEINES DE FUSION CONTENANT UNE PROTEINE REP DE VIRUS ASSOCIE A L'ADENOVIRUS ET PROTEINE BACTERIENNE

Publication

EP 0733122 A4 19980610 (EN)

Application

EP 94919252 A 19940526

Priority

  • US 9405940 W 19940526
  • US 6723693 A 19930526

Abstract (en)

[origin: WO9428157A1] A fusion protein including an adeno-associated virus rep protein or a fragment or derivative thereof, and a protein or peptide which is not an adeno-associated virus protein or peptide. Such fusion protein may be produced by genetic engineering techniques wherein there is provided an expression vehicle including a first DNA sequence encoding an adeno-associated virus rep protein or a fragment or derivative thereof, and a second DNA sequence encoding a protein or peptide which is not an adeno-associated virus protein or peptide, whereby expression of said first DNA sequence and said second DNA sequence results in expression of a fusion protein including the adeno-associated virus rep protein or fragment or derivative thereof and the protein or peptide, such as the E. coli maltose-binding protein, which is not an adeno-associated virus protein or peptide. Such fusion proteins may be produced and purified in large quantities while the adeno-associated virus rep protein portion of the fusion protein retains its biological activity.

IPC 1-7

C12P 21/06; C12N 9/14; C07K 1/00; C07K 14/00; C07K 15/00; C07K 17/00; A01N 43/04; C07H 17/00; C07H 19/00; C07H 21/00

IPC 8 full level

C12N 15/09 (2006.01); C07H 21/04 (2006.01); C07K 14/015 (2006.01); C12N 1/21 (2006.01); C12N 9/14 (2006.01); C12P 21/02 (2006.01); A61K 38/00 (2006.01); C12R 1/19 (2006.01)

CPC (source: EP)

C07K 14/005 (2013.01); C12N 9/14 (2013.01); A61K 38/00 (2013.01); C07K 2319/24 (2013.01); C07K 2319/40 (2013.01); C12N 2750/14122 (2013.01)

Citation (search report)

  • [A] EP 0486917 A2 19920527 - BEHRINGWERKE AG [DE]
  • [XY] IM D.S: & MUZYCZKA N.: "The AAV origin bvinding protein Rep68 is an ATP-dependent site-specific endonuclease with DNA helicase activity.", CELL, vol. 61, 4 May 1990 (1990-05-04), pages 447 - 457, XP002060793
  • [Y] NEW ENGLAND BIOLABS CATALOG, 1992, pages 96 - 97, XP002061117
  • [PX] CHIORINI J A ET AL: "BIOLOGICALLY ACTIVE REP PROTEINS OF ADENO-ASSOCIATED VIRUS TYPE 2 PRODUCED AS FUSION PROTEINS IN ESCHERICHIA COLI", JOURNAL OF VIROLOGY, vol. 68, no. 2, February 1994 (1994-02-01), pages 797 - 804, XP000608411
  • See references of WO 9428157A1

Designated contracting state (EPC)

AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 9428157 A1 19941208; CA 2162271 A1 19941208; EP 0733122 A1 19960925; EP 0733122 A4 19980610; JP H09501309 A 19970210

DOCDB simple family (application)

US 9405940 W 19940526; CA 2162271 A 19940526; EP 94919252 A 19940526; JP 50096595 A 19940526