Global Patent Index - EP 0734446 B1

EP 0734446 B1 20020717 - CONTROLLED EXPRESSION OF TRANSGENIC CONSTRUCTS IN PLANT PLASTIDS

Title (en)

CONTROLLED EXPRESSION OF TRANSGENIC CONSTRUCTS IN PLANT PLASTIDS

Title (de)

KONTROLLIERTE EXPRESSION TRANSGENER KONSTRUKTE IN PFLANZENPLASTIDEN

Title (fr)

EXPRESSION MAITRISEE DE PRODUITS D'ASSEMBLAGE TRANSGENIQUES DANS DES PLASTES

Publication

EP 0734446 B1 20020717 (EN)

Application

EP 95905422 A 19941214

Priority

  • US 9414574 W 19941214
  • US 16763893 A 19931214

Abstract (en)

[origin: US5576198A] Novel compositions and methods useful for genetic engineering of plant cells to provide a method of controlling the timing or tissue pattern of expression of foreign DNA sequences inserted into the plant plastid genome are provided in the instant invention. Constructs include those for nuclear transformation which provide for expression of a viral single subunit RNA polymerase in plant cell tissues, and targeting of the expressed polymerase protein into the plant cell plastids. In addition, plastid expression constructs comprising a viral gene promoter region which is specific to the RNA polymerase expressed from the nuclear expression constructs described above, and a DNA sequence of interest to be expressed in the transformed plastid cells are provided. Plant cells and plants comprising the nuclear and/or plastid constructs described herein are of interest. Of particular interest is a method of controlling expression of the inserted plastid gene constructs in a tissue and/or developmental specific manner in plants comprising both the nuclear polymerase construct and the plastid expression constructs.

IPC 1-7

C12N 15/82; A01H 5/00; C12N 5/10

IPC 8 full level

A01H 5/00 (2006.01); C12N 5/10 (2006.01); C12N 15/09 (2006.01); C12N 15/82 (2006.01)

CPC (source: EP US)

C12N 15/8214 (2013.01 - EP US)

Designated contracting state (EPC)

AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 9516783 A1 19950622; AT E220722 T1 20020815; DE 69430993 D1 20020822; DE 69430993 T2 20030320; EP 0734446 A1 19961002; EP 0734446 B1 20020717; JP 3670662 B2 20050713; JP H09510342 A 19971021; US 5576198 A 19961119

DOCDB simple family (application)

US 9414574 W 19941214; AT 95905422 T 19941214; DE 69430993 T 19941214; EP 95905422 A 19941214; JP 51699195 A 19941214; US 16763893 A 19931214