EP 0748374 A1 19961218 - METHOD FOR PREPARING CLONOGENIC FIBROBLASTS, METHOD FOR GENE TRANSFECTION OF FIBROBLASTS AND GENE-TRANSFECTED FIBROBLASTS SO OBTAINED
Title (en)
METHOD FOR PREPARING CLONOGENIC FIBROBLASTS, METHOD FOR GENE TRANSFECTION OF FIBROBLASTS AND GENE-TRANSFECTED FIBROBLASTS SO OBTAINED
Title (de)
VERFAHREN ZUR HERSTELLUNG VON KLONOGENEN FIBROBLASTEN, VERFAHREN ZUR GENTRANSFIZIERUNG VON FIBROBLASTEN UND SO ERHALTENE GENTRANSFIZIERTE FIBROBLASTEN
Title (fr)
MODE DE PREPARATION DE FIBROPLASTS CLONOGENES, METHODE DE TRANSFECTION GENIQUE DE FIBROPLASTS ET LES FIBROPLASTS GENOTRANSFECTES AINSI OBTENUS
Publication
Application
Priority
- DE 4406073 A 19940224
- EP 9500660 W 19950223
Abstract (en)
[origin: DE4406073A1] In a method for preparing clonogenic fibroblasts, tissue is taken from the donor and the cells isolated from the tissue, the cell suspension so obtained is sieved, the cells contained in the cell suspension are grown and the cells are transferred to a tissue culture; alternatively, the cells are isolated by mechanical comminution followed by enzymatic treatment with collagenase alone. As a result of the transfection into the fibroblasts, at least one gene which codes for a biologically active protein, preferably a therapeutically active protein such as a growth factor, a hormone, an enzyme, a clotting factor or a clotting inhibitor, is inwardly transferred.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); A61K 9/00 (2006.01); A61K 31/70 (2006.01); A61K 35/76 (2006.01); A61K 38/00 (2006.01); A61K 38/22 (2006.01); A61K 38/43 (2006.01); A61K 38/55 (2006.01); A61K 47/30 (2006.01); A61K 48/00 (2006.01); A61P 43/00 (2006.01); C07H 21/04 (2006.01); C07K 14/535 (2006.01); C12N 5/077 (2010.01); C12N 5/10 (2006.01); C12N 15/12 (2006.01)
CPC (source: EP US)
A61P 43/00 (2017.12 - EP); C07K 14/535 (2013.01 - EP US); C12N 5/0656 (2013.01 - EP US); A61K 38/00 (2013.01 - EP US); A61K 48/00 (2013.01 - EP US); C12N 2510/02 (2013.01 - EP US)
Citation (search report)
See references of WO 9523216A1
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
DE 4406073 A1 19950831; AU 1811795 A 19950911; CA 2183438 A1 19950831; EP 0748374 A1 19961218; JP H09509571 A 19970930; US 6093393 A 20000725; WO 9523216 A1 19950831
DOCDB simple family (application)
DE 4406073 A 19940224; AU 1811795 A 19950223; CA 2183438 A 19950223; EP 9500660 W 19950223; EP 95909773 A 19950223; JP 52213095 A 19950223; US 70050696 A 19960822