EP 0751989 A1 19970108 - ISOENZYME CALIBRATOR/CONTROL PRODUCTS
Title (en)
ISOENZYME CALIBRATOR/CONTROL PRODUCTS
Title (de)
ISOENZYME KALIBRATOR/KONTROLLPRODUKTE
Title (fr)
DISPOSITIF DE CALIBRAGE/CONTROLE D'ISOENZYMES
Publication
Application
Priority
- US 9501927 W 19950217
- US 19891994 A 19940218
Abstract (en)
[origin: WO9522602A1] The invention relates to buffers used to stabilize isoenzymes with formulations which upon drying and reconstitution recover essentially all of the isoenzyme activity and immunological mass for long term shelf-life storage without experiencing dissociation and rearrangement of its subunits. The buffer used for the long term dry storage of an isoenzyme is a serum-free and a salt-free aqueous buffer which comprises: 1) a glass-forming sugar; 2) an antioxidant mixture; 3) a pH buffer; and 4) a non-ionic surfactant and/or a synthetic polymer and/or a gelatin. The buffer used for the reconstitution of a serum-free and salt-free isoenzyme sample comprises an aqueous buffer containing a salt. In addition, a serum-isoenzyme is disclosed which comprises: 1) a pH buffer; 2) a salt; 3) an antioxidant mixture; and 4) a non-ionic surfactant and/or a synthetic polymer and/or a gelatin. The figure shows the accelerated stability of isoenzyme mass in a serum-based and a serum-free aqueous buffer as determined by an arrhenius plot of the days to reach stability failure against the reciprocal of temperature, extrapolating a storage shelf-life at 5 DEG C.
IPC 1-7
IPC 8 full level
CPC (source: EP)
C12N 9/96 (2013.01)
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9522602 A1 19950824; AU 1844895 A 19950904; AU 700666 B2 19990114; CA 2183573 A1 19950824; EP 0751989 A1 19970108; EP 0751989 A4 19990714; JP H09509064 A 19970916
DOCDB simple family (application)
US 9501927 W 19950217; AU 1844895 A 19950217; CA 2183573 A 19950217; EP 95910271 A 19950217; JP 52189695 A 19950217