EP 0755400 A1 19970129 - PURIFICATION OF OLIGODEOXYNUCLEOTIDE PHOSPHOROTHIOATES USING ANION EXCHANGE CHROMATOGRAPHY
Title (en)
PURIFICATION OF OLIGODEOXYNUCLEOTIDE PHOSPHOROTHIOATES USING ANION EXCHANGE CHROMATOGRAPHY
Title (de)
AUFREINIGUNG VON OLIGODESOXYNUKLEOTID-PHOSPHOROTHIOATEN DURCH ANIONENAUSTAUSCHCHROMATOGRAPHIE
Title (fr)
PURIFICATION DE PHOSPHOROTHIOATES D'OLIGODESOXYNUCLEOTIDES PAR CHROMATOGRAPHIE PAR ECHANGE D'ANIONS
Publication
Application
Priority
- US 9504492 W 19950407
- US 22547594 A 19940408
Abstract (en)
[origin: WO9527718A2] The present invention provides improved methods for separating and purifying oligonucleotide phosphorothioates. For the first time, we used DMAE ion-exchange chromatography to separate and purify oligonucleotide phosphorothioates. We found that by varying the conditions employed, excellent separation can be obtained. In particular, we have been able to use a higher pH and lower salt concentrations than previously reported in the literature to obtain chromatographic separation of oligonucleotide phosphorothioates having length up to about 35 nucleotides.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C07H 1/06 (2006.01); C07H 21/00 (2006.01); C07H 21/04 (2006.01); C12N 15/10 (2006.01)
CPC (source: EP)
C07H 21/00 (2013.01); C12N 15/101 (2013.01)
Citation (search report)
See references of WO 9527718A2
Citation (examination)
- JOURNAL OF CHROMATOGRAPHY, vol. 599, no. 1-2, 1992 AMSTERDAM NL, pages 35-42,
- BERGOT, B. JOHN ET AL 'Separation of synthetic phosphorothioate oligodeoxynucloetides from their oxygenated (phosphodiester) defect species by strong-anion-exchange high-performance liquid chromatography'
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9527718 A2 19951019; WO 9527718 A3 19951102; AU 2286795 A 19951030; CA 2187338 A1 19951019; CN 1150431 A 19970521; EP 0755400 A1 19970129; JP H10502052 A 19980224
DOCDB simple family (application)
US 9504492 W 19950407; AU 2286795 A 19950407; CA 2187338 A 19950407; CN 95193486 A 19950407; EP 95916332 A 19950407; JP 52651995 A 19950407