EP 0755444 A4 19980114 - MOLECULAR CLONING AND EXPRESSION OF A -g(g)-INTERFERON INDUCIBLE ACTIVATOR OF THE PROTEASOME
Title (en)
MOLECULAR CLONING AND EXPRESSION OF A -g(g)-INTERFERON INDUCIBLE ACTIVATOR OF THE PROTEASOME
Title (de)
MOLEKULARE KLONIERUNG UND EXPRESSION DES AKTIVATORS VON DEM DURCH A -g(g)-INTERFERON INDUZIERBAREN PROTEASOM
Title (fr)
CLONAGE MOLECULAIRE ET EXPRESSION D'UN ACTIVATEUR DU PROTEASOME INDUCTIBLE PAR L'INTERFERON GAMMA
Publication
Application
Priority
US 9403591 W 19940401
Abstract (en)
[origin: WO9527058A1] Molecular cloning and expression of a human gene encoding a polypeptide activator of proteasomes is disclosed. The expressed activator has an Mr of about 29,000 and is functional in activating proteasomes in vitro. In vivo this activator polypeptide is inducible with gamma -interferon in HeLa cells and occurs with a non- gamma -interferon-inducible polypeptide with an Mr of about 31,000 in a hexameric activator complex. The activator protein contains a lysine and glutamate rich region termed a KEKE motif. The KEKE motif appears to promote association between proteins and selection of peptides for presentation on MHC Class I receptors. A method for enhancing cell-mediated immunity against or tolerance to a selected immunogenic peptide is described comprising expressing activator and the selected peptide, wherein the selected peptide is adjacent to a KEKE motif, in an appropriate cell.
IPC 1-7
C12N 15/12; C12N 15/09; C12N 9/64; C07K 14/47; A61K 39/385; A61K 39/39; A61K 48/00
IPC 8 full level
C12N 9/64 (2006.01); C12N 15/12 (2006.01); A61K 39/00 (2006.01)
CPC (source: EP)
C12N 9/6421 (2013.01); A61K 39/00 (2013.01)
Citation (search report)
[T] C. REALINI ET AL.: "Molecular cloning and expression of a gamma-interferon- inducible activator of the multicatalytic protease", J. BIOL. CHEM., vol. 269, no. 2, 12 August 1994 (1994-08-12), pages 20727 - 32, XP002046903
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9527058 A1 19951012; AU 7353094 A 19951023; EP 0755444 A1 19970129; EP 0755444 A4 19980114
DOCDB simple family (application)
US 9403591 W 19940401; AU 7353094 A 19940401; EP 94922414 A 19940401