EP 0760102 A1 19970305 - ISOLATION OF HEMATOPOIETIC DENDRITIC CELLS BY HIGH GRADIENT MAGNETIC CELL SORTING
Title (en)
ISOLATION OF HEMATOPOIETIC DENDRITIC CELLS BY HIGH GRADIENT MAGNETIC CELL SORTING
Title (de)
ISOLIERUNG HEMATOPOIETISCHER DENDRITISCHER ZELLEN DURCH MAGNETISCHE ZELLSORTIERUNG MIT HOHEM GRADIENTEN
Title (fr)
ISOLEMENT DE CELLULES DENDRITIQUES HEMATOPOIETIQUES PAR TRIAGE MAGNETIQUE DE CELLULES A GRADIENT ELEVE
Publication
Application
Priority
- US 9603265 W 19960311
- US 40336295 A 19950315
Abstract (en)
[origin: WO9628732A1] Methods are provided for the rapid isolation of highly purified and functionally intact dendritic cells from a mixed cell population, using colloidal superparamagnetic particles. Dendritic cells are enriched from a blood or lymph sample using a two step high-gradient magnetic cell separation. Lymphocytes, natural killer and monocytic cells are depleted by specific binding to markers present on lymphoid and myeloid cells. In a separate step, dendritic cells are enriched by HGMS. Purified dendritic cells are useful as a source of antigen presenting cells for in vitro analysis, and in immunomodulating therapies, particularly for priming naive T cells.
IPC 1-7
IPC 8 full level
G01N 33/53 (2006.01); G01N 33/553 (2006.01); G01N 33/569 (2006.01)
CPC (source: EP)
G01N 33/56972 (2013.01); G01N 33/56977 (2013.01)
Citation (search report)
See references of WO 9628732A1
Designated contracting state (EPC)
AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9628732 A1 19960919; AU 5306496 A 19961002; CA 2190367 A1 19960919; EP 0760102 A1 19970305; JP H10500492 A 19980113
DOCDB simple family (application)
US 9603265 W 19960311; AU 5306496 A 19960311; CA 2190367 A 19960311; EP 96909637 A 19960311; JP 52776196 A 19960311