EP 0765395 A1 19970402 - N-TERMINALLY EXTENDED PROTEINS EXPRESSED IN YEAST
Title (en)
N-TERMINALLY EXTENDED PROTEINS EXPRESSED IN YEAST
Title (de)
AM N-TERMINUS VERLÄNGERTE PROTEINE DIE IN HEFE EXPRIMIERT WERDEN
Title (fr)
PROTEINES COMPORTANT UNE EXTENSION A L'EXTREMITE N-TERMINALE, EXPRIMEES PAR UNE LEVURE
Publication
Application
Priority
- DK 9500250 W 19950616
- DK 71294 A 19940617
Abstract (en)
[origin: WO9535384A1] The present invention relates to polypeptides expressed and processed in yeast, a DNA construct comprising a DNA sequence encoding polypeptide having the following structure signal peptide-leader peptide -X1-X2-X3-X4-X5-X6-X7- heterologous protein, X1 is Lys or Arg; X2 is Lys or Arg, X1 and X2 together defining a yeast processing site; X3 is Glu or Asp; X4 is a sequence of amino acids with the following structure (A-B)n wherein A is Glu or Asp, B is Ala, Val, Leu or Pro, and n is 0 or an integer from 1 to 5, and when n > 2 each A and B is the same or different from the other A(s) and B(s), or X4 is a sequence of amino acids with the following structure (C)m wherein C is Glu or Asp, and m is 0 or an integer from 1 to 5; X5 is a peptide bond or is one or more amino acids which may be the same or different; X6 is a peptide bond or an amino acid residue selected from the group consisting of Pro, Asp, Thr, Ser, Glu, Ala and Gly; and X7 is Lys or Arg.
IPC 1-7
IPC 8 full level
C12N 15/09 (2006.01); C07K 14/62 (2006.01); C12N 1/19 (2006.01); C12N 15/62 (2006.01); C12N 15/81 (2006.01); C12P 21/02 (2006.01); C12R 1/865 (2006.01)
CPC (source: EP)
C07K 14/62 (2013.01); C12N 15/62 (2013.01); C12N 15/81 (2013.01); C07K 2319/00 (2013.01); C07K 2319/02 (2013.01)
Citation (search report)
See references of WO 9535384A1
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IE IT LI LU NL PT SE
DOCDB simple family (publication)
WO 9535384 A1 19951228; AU 2733595 A 19960115; BR 9508053 A 19970812; CA 2192943 A1 19951228; CN 1131312 C 20031217; CN 1152942 A 19970625; CZ 363796 A3 19970514; EP 0765395 A1 19970402; FI 965030 A0 19961216; FI 965030 A 19970214; HU 9603476 D0 19970228; HU T75840 A 19970528; IL 114160 A0 19951031; IL 114160 A 20061231; JP 3730255 B2 20051221; JP H10501695 A 19980217; MX 9606536 A 19970329; NO 965411 D0 19961216; NO 965411 L 19970214; PL 317761 A1 19970428; SG 47883 A1 19980417; ZA 954983 B 19960214
DOCDB simple family (application)
DK 9500250 W 19950616; AU 2733595 A 19950616; BR 9508053 A 19950616; CA 2192943 A 19950616; CN 95194181 A 19950616; CZ 363796 A 19950616; EP 95922440 A 19950616; FI 965030 A 19961216; HU 9603476 A 19950616; IL 11416095 A 19950615; JP 50150596 A 19950616; MX 9606536 A 19950616; NO 965411 A 19961216; PL 31776195 A 19950616; SG 1996004970 A 19950916; ZA 954983 A 19950615