EP 0766697 A1 19970409 - METHOD OF ISOLATING ISOLECTINS FROM MISTLETOE
Title (en)
METHOD OF ISOLATING ISOLECTINS FROM MISTLETOE
Title (de)
VERFAHREN ZUR ISOLIERUNG VON ISOLEKTINEN AUS DER MISTEL
Title (fr)
PROCEDE PERMETTANT D'ISOLER DES ISOLECTINES DE GUI
Publication
Application
Priority
- DE 4421895 A 19940623
- EP 9502445 W 19950623
Abstract (en)
[origin: DE4421895A1] The invention concerns a method of isolating type ML I isolectins from mistletoe, the method calling for the following operations: mistletoe plants freshly harvested from birch or sycamore host plants are chopped and an aqueous suspension made; the aqueous suspension is optionally filtered and its pH adjusted to < 7; the suspension is optionally centrifuged, the supernatant liquid passed through a cation-exchange column and the eluting fraction containing the lectins from the mistletoe collected; this fraction is subjected to chromatography using a lactosyl-modified column support material; and the type ML I lectins are desorbed by treating the lactosyl-modified support material and separated on a cation-exchange column and from which the type ML I-1 and ML I-2 fractions are collected. The invention also concerns type ML I-1 and ML I-2 mistletoe lectins obtained by a method as described in one of claims 1 to 5, plus fragments obtained by reductive splitting with disulphide-bond-splitting reagents.
IPC 1-7
IPC 8 full level
G01N 33/53 (2006.01); C07K 1/18 (2006.01); C07K 1/36 (2006.01); C07K 14/42 (2006.01); C07K 16/16 (2006.01); C12P 21/08 (2006.01)
CPC (source: EP)
C07K 1/36 (2013.01); C07K 14/42 (2013.01); C07K 16/16 (2013.01)
Citation (search report)
See references of WO 9600239A1
Designated contracting state (EPC)
BE CH DE DK ES FR GB IT LI NL SE
DOCDB simple family (publication)
DE 4421895 A1 19960104; AU 2887595 A 19960119; EP 0766697 A1 19970409; JP H10504287 A 19980428; WO 9600239 A1 19960104
DOCDB simple family (application)
DE 4421895 A 19940623; AU 2887595 A 19950623; EP 9502445 W 19950623; EP 95924317 A 19950623; JP 50279896 A 19950623