EP 0767834 A1 19970416 - ANTISENSE SEQUENCE AND RIBOZYME EXPRESSION CASSETTE
Title (en)
ANTISENSE SEQUENCE AND RIBOZYME EXPRESSION CASSETTE
Title (de)
EXPRESSIONSKASSETTE FÜR DIE ANTISENSE- UND DIE RIBOZYM-EXPRESSION
Title (fr)
CASSETTE D'EXPRESSION DE SEQUENCES ANTISENS ET DE RIBOZYMES
Publication
Application
Priority
- DE 9500663 W 19950519
- DE 4424761 A 19940704
- US 31458894 A 19940928
Abstract (en)
[origin: DE4424761C1] The object of the invention is to construct a vector for antisense and ribozyme expression capable of expressing a determined and desired ribozyme or antisense sequence in a cell in a continuous and stable manner. The invention has applications in molecular biology and genetic engineering. The disclosed expression cassette is characterised in that it contains a strong promoter, preferably a T7 promoter, an adenoviral va-RNA-gene, a stable loop region and an insertion site for antisense/ribozyme sequences in the loop region. The T7 promoter is preferably associated with a T7 polymerase. The loop region is located in a restriction site in the central part of the adenoviral va-RNA gene and it preferably contains at least 2x21 bases having the same sequence.
IPC 1-7
IPC 8 full level
C07K 14/61 (2006.01); C12N 15/11 (2006.01); C12N 15/113 (2010.01)
CPC (source: EP US)
C07K 14/61 (2013.01 - EP US); C12N 15/113 (2013.01 - EP US)
Citation (search report)
See references of WO 9601315A1
Designated contracting state (EPC)
BE CH DK FR GB IT LI NL SE
DOCDB simple family (publication)
DE 4424761 C1 19950608; EP 0767834 A1 19970416; US 5695992 A 19971209; US RE37411 E 20011016; WO 9601315 A1 19960118
DOCDB simple family (application)
DE 4424761 A 19940704; DE 9500663 W 19950519; EP 95919314 A 19950519; US 31458894 A 19940928; US 8959498 A 19980602