EP 0776374 B1 20091216 - PROCESS AND DEVICE FOR DETERMINING THE ACTIVITY OF ENZYMES IN LIQUIDS, OR THE CONCENTRATION AND/OR ACTIVITY OF INHIBITORS IN LIQUIDS
Title (en)
PROCESS AND DEVICE FOR DETERMINING THE ACTIVITY OF ENZYMES IN LIQUIDS, OR THE CONCENTRATION AND/OR ACTIVITY OF INHIBITORS IN LIQUIDS
Title (de)
VERFAHREN UND VORRICHTUNG ZUR BESTIMMUNG DER AKTIVITÄT VON ENZYMEN IN FLÜSSIGKEITEN, BEZIEHUNGSWEISE DER KONZENTRATION UND/ODER AKTIVITÄT VON INHIBITOREN IN FLÜSSIGKEITEN
Title (fr)
PROCEDE ET DISPOSITIF DE DETERMINATION DE L'ACTIVITE D'ENZYMES DANS DES LIQUIDES OU DE LA CONCENTRATION ET/OU ACTIVITE D'INHIBITEURS DANS DES LIQUIDES
Publication
Application
Priority
- DE 9601087 W 19960619
- DE 19522255 A 19950620
- DE 19527880 A 19950729
- DE 19617731 A 19960503
Abstract (en)
[origin: WO9700969A1] A process and device are disclosed to determine the activity of enzymes in liquids in a largely automatic manner. The device for carrying out this process has a column (1) with a chromatographic carrier for treating a measurement sample. The carrier is mixed with a substance capable of binding to an enzyme inhibitor present in the measurement sample and that corresponds to at least one enzyme. A measurement sample supply (2) is associated to one end of the column (1). A valve/pump arrangement (7, 11, 14, 15) for filling at least one test tube (5) with a carrier and at least part of the measurement sample is connected downstream of the column (1), in the flow direction of the measurement sample. The carrier is dissociated into cleavage products by the action of the enzyme. The rise in concentration per unit of time of at least one of the cleavage products of the carrier is sensed during an incubation time. As an alternative or supplementary step, the enzyme that corresponds to at least one enzyme inhibitor is extracted by chromatography from a measurement sample to detect enzyme inhibitors in liquids and the thus treated measurement sample is tested for inhibitor concentration and/or activity.
IPC 8 full level
CPC (source: EP US)
C12M 41/46 (2013.01 - EP US); C12Q 1/00 (2013.01 - EP US)
Citation (examination)
- US 3983001 A 19760928 - COUPEK JIRI, et al
- Koohmaraie; Kretchar (1990) J. Anim. Sci. 68, 2362-2370.
- Anastasi et al: (1983) Biochem. J. 211, 129-138.
- Afting; Becker: (1981) Biochem. J. 197, 519-522
- Schroeder et al: (1977) J. Chromatography 134, 83-90
- de Jong; Kwakman: (1989) J. Chromatography 492, 319-343
- Brinkman et al: (1989) J. Chromatography 492, 251-298
Designated contracting state (EPC)
CH DE FR GB IT LI NL SE
DOCDB simple family (publication)
WO 9700969 A1 19970109; AU 6187296 A 19970122; AU 708196 B2 19990729; CA 2229986 A1 19970109; CA 2229986 C 20141021; EP 0776374 A1 19970604; EP 0776374 B1 20091216; US 2001034057 A1 20011025; US 2003087426 A1 20030508; US 2008268464 A1 20081030; US 5935846 A 19990810; US 6171851 B1 20010109; US 7368282 B2 20080506
DOCDB simple family (application)
DE 9601087 W 19960619; AU 6187296 A 19960619; CA 2229986 A 19960619; EP 96920690 A 19960619; US 10972508 A 20080425; US 31653999 A 19990521; US 32662602 A 20021219; US 75375301 A 20010103; US 79383397 A 19970218