EP 0777741 A4 19990113 - BIOSENSOR FOR AND METHOD OF ELECTROGENERATED CHEMILUMINESCENT DETECTION OF NUCLEIC ACID ADSORBED TO A SOLID SURFACE
Title (en)
BIOSENSOR FOR AND METHOD OF ELECTROGENERATED CHEMILUMINESCENT DETECTION OF NUCLEIC ACID ADSORBED TO A SOLID SURFACE
Title (de)
VERFAHREN ZUM NACHWEIS VON AUF EINER FESTEN OBERFLÄCHE ADSORBIERTER NUKLEINSÄURE DURCH ELEKTROGENERIERTE CHEMILUMINESZENZ UND BIOSENSOR DAFÜR
Title (fr)
BIOCAPTEUR ET PROCEDE DESTINES A LA DETECTION PAR LUMINESCENCE ELECTROPHOTOCHIMIQUE D'ACIDES NUCLEIQUES ADSORBES SUR UNE SURFACE SOLIDE
Publication
Application
Priority
- US 9510630 W 19950825
- US 29663094 A 19940826
Abstract (en)
[origin: WO9606946A1] Single-strand DNA was immobilized on an electrode covered with an aluminum alkanebisphosphonate film by immersing it in an ss-DNA solution. The immobilized ss-DNA labeled with Ru(bpy)3<2+> was detected by monitoring the electrogenerated chemiluminescence (ECL) produced upon oxidation in a solution containing tri-n-propylamine. After immobilization of unlabeled ss-DNA, a complementary labeled strand DNA was hybridized to produce ds-DNA on the surface. The extent of DNA hybridization was determined by ECL of the labeled Ru(bpy)3<2+>. Surface immobilized ds-DNA could also be detected by observing the ECL of intercalated Ru(phen)3<2+>. Transmission electron microscopy (TEM) was employed to image the film and the immobilized DNA.
IPC 1-7
C12Q 1/00; C12Q 1/70; C12Q 1/68; C12P 19/34; C07H 21/04; C25D 11/12; G01N 33/53
IPC 8 full level
G01N 33/483 (2006.01); C12N 15/09 (2006.01); C12Q 1/00 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6816 (2018.01); C12Q 1/6825 (2018.01); C12Q 1/6869 (2018.01); G01N 21/76 (2006.01); G01N 21/78 (2006.01); G01N 33/543 (2006.01); G01N 33/58 (2006.01); G01N 37/00 (2006.01)
CPC (source: EP)
C12Q 1/005 (2013.01); C12Q 1/6816 (2013.01); C12Q 1/6825 (2013.01); C12Q 1/6869 (2013.01); G01N 33/582 (2013.01)
Citation (search report)
- No further relevant documents disclosed
- See references of WO 9606946A1
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9606946 A1 19960307; AU 3410395 A 19960322; AU 703344 B2 19990325; CA 2198489 A1 19960307; EP 0777741 A1 19970611; EP 0777741 A4 19990113; JP H10509025 A 19980908
DOCDB simple family (application)
US 9510630 W 19950825; AU 3410395 A 19950825; CA 2198489 A 19950825; EP 95930881 A 19950825; JP 50882296 A 19950825