Global Patent Index - EP 0778899 A4

EP 0778899 A4 20010829 - ENRICHMENT OF FETAL CELLS FROM MATERNAL BLOOD

Title (en)

ENRICHMENT OF FETAL CELLS FROM MATERNAL BLOOD

Title (de)

ANREICHERUNG VON FÖTALEN ZELLEN AUS MATERNALEM BLUT

Title (fr)

ENRICHISSEMENT DE CELLULES F TALES, A PARTIR DU SANG MATERNEL

Publication

EP 0778899 A4 20010829 (EN)

Application

EP 95933174 A 19950919

Priority

  • US 9511971 W 19950919
  • US 30922994 A 19940920

Abstract (en)

[origin: WO9609409A1] Methods of preparing fetal cells from samples of maternal peripheral blood are provided. A suspension of mononuclear cells is prepared from a maternal blood sample. Maternal cells are then depleted from the sample by adding magnetically coupled antibodies specific for markers present on adult cells, followed by binding the cells to a column in the presence of a magnetic field. From the depleted fraction, fetal erythrocytes are enriched by magnetic cell sorting (MACS). These nucleated erythrocytes are then used as a source of fetal genetic material, which is analyzed for the presence of chromosomal or genetic abnormalities, or for the presence of a Y chromosome, by conventional methods such as probes for in situ hybridization, metaphase spreads, and polymerase chain reaction (PCR).

IPC 1-7

C12Q 1/68; G01N 33/569

IPC 8 full level

G01N 33/50 (2006.01); C12N 15/09 (2006.01); C12Q 1/68 (2006.01); C12Q 1/6804 (2018.01); G01N 33/53 (2006.01); G01N 33/543 (2006.01); G01N 33/553 (2006.01); G01N 33/569 (2006.01); G01N 33/577 (2006.01); G01N 33/68 (2006.01)

CPC (source: EP)

C12Q 1/6804 (2013.01); G01N 33/54326 (2013.01); G01N 33/56972 (2013.01); G01N 33/689 (2013.01); G01N 2800/368 (2013.01)

Citation (search report)

  • [Y] WO 9116452 A1 19911031 - CELLPRO INC [US]
  • [X] BÜSCH J ET AL.: "Simple and fast 'Double-MACS' sorting of fetal erythroblasts from maternal blood for PCR-based paternity analysis", ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, vol. 731, 1994, pages 144 - 146, XP001011400
  • [DX] GÄNSHIRT-AHLERT D ET AL.: "Magnetic cell sorting and the transferrin receptor as potential means of prenatal diagnosis from maternal blood", AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, vol. 166, May 1992 (1992-05-01), pages 1350 - 1355, XP001011368
  • [DY] MILTENYI S ET AL: "HIGH GRADIENT MAGNETIC CELL SEPERATION WITH MACS", CYTOMETRY,ALAN LISS, NEW YORK,US, vol. 11, no. 11, 1990, pages 231 - 238, XP000999711, ISSN: 0196-4763
  • [DA] CHUEH J AND GOLBUS M S: "Prenatal diagnosis using fetal cells in the maternal circulation", SEMINARS IN PERINATOLOGY, vol. 14, no. 6, 1990, pages 471 - 482, XP001011366
  • [A] HOLZGREVE W ET AL.: "Noninvasive prenatal diagnosis", ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, vol. 731, 1994, pages 253 - 256, XP001011401
  • [PX] READING J P ET AL.: "Nucleated erythrocytes in maternal blood: quantity and quality of fetal cells in enriched populations", HUMAN REPRODUCTION, vol. 10, no. 9, September 1995 (1995-09-01), pages 2510 - 2515, XP001011367
  • See references of WO 9609409A1

Designated contracting state (EPC)

AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE

DOCDB simple family (publication)

WO 9609409 A1 19960328; AU 3593595 A 19960409; AU 691040 B2 19980507; CA 2200294 A1 19960328; EP 0778899 A1 19970618; EP 0778899 A4 20010829; JP H10507632 A 19980728

DOCDB simple family (application)

US 9511971 W 19950919; AU 3593595 A 19950919; CA 2200294 A 19950919; EP 95933174 A 19950919; JP 51105895 A 19950919