EP 0781337 A1 19970702 - HUMANISED ANTIBODY TO INTEGRIN CHAIN BETA-1
Title (en)
HUMANISED ANTIBODY TO INTEGRIN CHAIN BETA-1
Title (de)
HUMANISIERTER ANTIKÖRPER GEGEN DIE BETA 1 KETTE VON INTEGRINEN
Title (fr)
ANTICORPS HUMANISE DIRIGE CONTRE LA CHAINE BETA 1 DES INTEGRINES
Publication
Application
Priority
- FR 9501167 W 19950912
- FR 9410858 A 19940912
Abstract (en)
[origin: WO9608564A1] Variable domains of a non-human immunoglobulin are humanised by selecting DNA sequences coding for the variable domains of a human immunoglobulin wherein the amino acid sequence of the framework regions of variable domains VH and VL is 70-95 % homologous with the amino acid sequence of the framework regions of variable domains VH and VL of non-human immunoglobulin, and wherein hypervariable loops H1 and H2 or L1, L2 and L3 have the same kind of canonical structure as hypervariable loops H1 and H2 or L1, L2 and L3, respectively, of non-human immunoglobulin, and by replacing the oligonucleotide sequences coding for the CDRs of human immunoglobulin with oligonucleotide sequences coding for the corresponding CDRs of non-human immunoglobulin. The cloning, in a baculovirus, of DNA fragments that code for said humanised variable domains, and the expression of the resulting humanised recombinant antibody, are also disclosed. Murine monoclonal antibody K20 has thus been humanised and expressed according to the Invention.
IPC 1-7
IPC 8 full level
C07K 16/00 (2006.01); C07K 16/28 (2006.01); C12N 15/866 (2006.01); C12P 21/08 (2006.01); A61K 38/00 (2006.01)
CPC (source: EP)
C07K 16/00 (2013.01); C07K 16/2842 (2013.01); C12N 15/86 (2013.01); A61K 38/00 (2013.01); C12N 2710/14143 (2013.01)
Citation (search report)
See references of WO 9608564A1
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9608564 A1 19960321; CA 2199749 A1 19960321; EP 0781337 A1 19970702; FR 2724393 A1 19960315
DOCDB simple family (application)
FR 9501167 W 19950912; CA 2199749 A 19950912; EP 95930574 A 19950912; FR 9410858 A 19940912