EP 0781411 A1 19970702 - SEPARATION OF NUCLEIC ACIDS BY CAPILLARY ELECTROPHORESIS IN THERMAL GRADIENTS IN VISCOUS POLYMER SOLUTIONS
Title (en)
SEPARATION OF NUCLEIC ACIDS BY CAPILLARY ELECTROPHORESIS IN THERMAL GRADIENTS IN VISCOUS POLYMER SOLUTIONS
Title (de)
TRENNUNG VON NUKLEINSÄUREN DURCH KAPILLARELEKTROFORESE MIT THERMISCHEN GRADIENTEN IN VISKOSEN POLYMERLÖSUNGEN
Title (fr)
SEPARATION D'ACIDES NUCLEIQUES DANS DES SOLUTIONS POLYMERES VISQUEUSES AU MOYEN D'UNE ELECTROPHORESE CAPILLAIRE A GRADIENTS THERMIQUES
Publication
Application
Priority
- EP 9503561 W 19950911
- IT MI941864 A 19940912
Abstract (en)
[origin: WO9608715A1] The present invention refers to the use of temporal thermal gradients for the separation of DNA fragments, amplified by PCR, both normal and containing point mutations, by capillary zone electrophoresis in presence of viscous polymer solutions. In this system the temperature control in the capillary is obtained via dedicated software and the fragments are revealed either by their natural UV absorbance at 254 nm or by laser induced fluorescence. It is also possible to operate with viscous solutions of polyacrylamides, in particular polyacrylamides containing N-substituted monomers, such as N-methylacrylamide and N-acryloyl amino ethoxy ethanol. Methods are described for producing short-chain polyacrylamides of low viscosity, which can be replaced after each run.
IPC 1-7
IPC 8 full level
G01N 27/447 (2006.01)
IPC 8 main group level
CPC (source: EP)
G01N 27/44704 (2013.01); G01N 27/44734 (2013.01)
Designated contracting state (EPC)
CH DE FR GB IT LI SE
DOCDB simple family (publication)
WO 9608715 A1 19960321; AU 3565295 A 19960329; AU 689648 B2 19980402; EP 0781411 A1 19970702; IT 1271005 B 19970526; IT MI941864 A0 19940912; IT MI941864 A1 19960312; JP 3015106 B2 20000306; JP H10502738 A 19980310
DOCDB simple family (application)
EP 9503561 W 19950911; AU 3565295 A 19950911; EP 95932707 A 19950911; IT MI941864 A 19940912; JP 50989296 A 19950911