EP 0782614 A1 19970709 - DIRECT INTRODUCTION OF FOREIGN MATERIALS INTO CELLS
Title (en)
DIRECT INTRODUCTION OF FOREIGN MATERIALS INTO CELLS
Title (de)
DIREKTES EINBRINGEN VON FREMDEN MATERIALIEN IN ZELLEN
Title (fr)
INTRODUCTION DIRECTE DE SUBSTANCES ETRANGERES DANS DES CELLULES
Publication
Application
Priority
- US 9512381 W 19950929
- US 31533694 A 19940930
- US 33460694 A 19941104
Abstract (en)
[origin: WO9610630A1] Disclosed is a simple, economical, and precise method of introducing a biological material into a predetermined target cell population. The method comprises the steps of providing (a) a plurality of biologically inert microprobes positioned on a support, (b) a solid or quasi-solid mass of the target cells defining an interface with the microprobes, and (c) a biological material at the interface, and then physically contacting the cells with the microprobes to cause the microprobes to non-lethally pierce the cell walls and/or membranes of the cells. The microprobes are preferably integral with the support, and are prepared by etching a single crystalline wafer material such as silicon. The mircoprobes are preferably pyramidally shaped. The target cells can be contacted with the microprobes in vitro or in situ. The method is applicable to virtually all cell types, and any biological material capable of being introduced into cells described herein.
IPC 1-7
C12N 5/00; C12N 15/06; C12N 13/00; C12M 1/04; C12M 3/04; C12N 15/85; C12N 15/82; A01K 29/00
IPC 8 full level
A01K 67/033 (2006.01); C07K 14/435 (2006.01); C12M 3/00 (2006.01); C12N 15/87 (2006.01); C12N 15/89 (2006.01)
CPC (source: EP)
A01K 67/0336 (2013.01); C07K 14/43545 (2013.01); C12M 35/00 (2013.01); C12N 15/87 (2013.01); C12N 15/89 (2013.01); C07K 2319/00 (2013.01)
Citation (search report)
See references of WO 9610630A1
Designated contracting state (EPC)
AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE
DOCDB simple family (publication)
WO 9610630 A1 19960411; AU 3758595 A 19960426; CA 2201315 A1 19960411; EP 0782614 A1 19970709
DOCDB simple family (application)
US 9512381 W 19950929; AU 3758595 A 19950929; CA 2201315 A 19950929; EP 95935656 A 19950929