Global Patent Index - EP 0784694 A1

EP 0784694 A1 19970723 - ENZYMATIC METHODS FOR POLYUNSATURATED FATTY ACID ENRICHMENT

Title (en)

ENZYMATIC METHODS FOR POLYUNSATURATED FATTY ACID ENRICHMENT

Title (de)

ENZYMATISCHE VERFAHREN ZUR ANREICHERUNG DER FETTE AN POLYUNGESÄTTIGTEN FETTSÄUREN

Title (fr)

PROCEDES ENZYMATIQUES D'ENRICHISSEMENT EN ACIDES GRAS POLYINSATURES

Publication

EP 0784694 A1 19970723 (FR)

Application

EP 96904125 A 19960213

Priority

  • FR 9600226 W 19960213
  • FR 9502153 A 19950224

Abstract (en)

[origin: WO9626287A1] A method for use in the field of chemistry and particularly in the chemistry of fats. The method is used to prepare polyunsaturated fatty acid concentrates and comprises selectively enzymatically hydrolysing a fish oil containing docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) in position 1, 2 or 3, to give a mixture of free fatty acids, monoglycerides and diglycerides, separating the components of the mixture, recovering the free fatty acids and purifying them by urea crystallisation to increase the EPA and DHA content, decomplexing the isolated fatty acids, and performing interesterification between the free fatty acids having a high polyunsaturated fatty acid concentration and the unprocessed oil, in the presence of a position- or steric hindrance-specific lipase, to give a polyunsaturated fatty acid triglyceride-enriched mixture which is separated while the free fatty acids are removed therefrom. A method for enzymatically enriching phospholipids with polyunsaturated fatty acids (EPA, DHA), and the synthesis of polyunsaturated fatty acid monoacylglycerols of series n3 by enzymatic synthesis using 1,2-dialkylene glycerol as the starting material, are also disclosed. The resulting products are suitable for use in foodstuffs, cosmetics and pharmaceuticals.

IPC 1-7

C12P 7/64; C11C 1/04; C11C 3/02; C11C 3/10

IPC 8 full level

C12P 7/6432 (2022.01); C11C 1/04 (2006.01); C11C 3/02 (2006.01); C11C 3/10 (2006.01); C12P 7/6434 (2022.01); C12P 7/6458 (2022.01); C12P 7/6472 (2022.01); C12P 7/6481 (2022.01)

CPC (source: EP US)

C11C 1/045 (2013.01 - EP US); C11C 3/02 (2013.01 - EP US); C11C 3/10 (2013.01 - EP US); C12P 7/6432 (2022.01 - EP US); C12P 7/6434 (2022.01 - EP US); C12P 7/6458 (2022.01 - EP US); C12P 7/6472 (2013.01 - EP US); C12P 7/6481 (2013.01 - EP US)

Citation (search report)

  • [A] WO 9108676 A1 19910627 - GEN FOODS INC [US]
  • [PX] WO 9524459 A1 19950914 - NORSK HYDRO AS [NO], et al
  • [A] DATABASE WPI Section Ch Week 199010, Derwent World Patents Index; Class D16, AN 1990-071781, XP002153638
  • [A] TSUNEO YAMANE ET AL: "PRODUCTION OF N-3 POLYUNSATURATED FATTY ACID-ENRICHED FISH OIL BY LIPASE-CATALYZED ACIDOLYSIS WITHOUT SOLVENT", JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY,US,AMERICAN OIL CHEMISTS' SOCIETY. CHAMPAIGN, vol. 69, no. 11, 1 November 1992 (1992-11-01), pages 1104 - 1107, XP000324894, ISSN: 0003-021X
  • [A] RATNAYAKE W M N ET AL: "PREPARATION OF OMEGA-3 PUFA CONCENTRATES FROM FISH OILS VIA UREA COMPLEXATION", FETT WISSENSCHAFT TECHNOLOGIE, vol. 90, no. 10, 1988, pages 381 - 386, XP002153633, ISSN: 0931-5985
  • [A] HAAGSMA N ET AL: "PREPARATION OF AN OMEGA-3 FATTY-ACID CONCENTRATE FROM COD LIVER OIL", JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY, vol. 59, no. 3, 1982, pages 117 - 118, XP000020563, ISSN: 0003-021X
  • [A] HARALDSON G G ET AL: "THE PREPARATION OF TRIGLYCERIDES HIGHLY ENRICHED WITH OMEGA-3 POLYUNSATURATED FATTY ACID VIA LIPASE CATALYZED INTERESTERIFICATION", TETRAHEDRON LETTERS, vol. 30, no. 13, 1989, pages 1671 - 1674, XP002153635, ISSN: 0040-4039
  • See also references of WO 9626287A1

Designated contracting state (EPC)

DE FR

DOCDB simple family (publication)

WO 9626287 A1 19960829; DZ 1997 A1 20020720; EP 0784694 A1 19970723; FR 2731015 A1 19960830; FR 2731015 B1 19970530; IL 117251 A0 19960618; IL 117251 A 20000928; MA 23806 A1 19961001; MY 115719 A 20030830; PE 51497 A1 19971224; TN SN96031 A1 20050315; US 6537787 B1 20030325

DOCDB simple family (application)

FR 9600226 W 19960213; DZ 960038 A 19960224; EP 96904125 A 19960213; FR 9502153 A 19950224; IL 11725196 A 19960223; MA 24163 A 19960215; MY PI9600665 A 19960223; PE 00026496 A 19960416; TN SN96031 A 19960221; US 73709898 A 19980313