EP 0786468 B1 20041013 - Solid support reagents for the direct synthesis of 3'-labeled polynucleotides
Title (en)
Solid support reagents for the direct synthesis of 3'-labeled polynucleotides
Title (de)
Festträger-Reagenz für Synthese von 3'-labilierte Polynukleotiden
Title (fr)
Réactifs sur supports solides pour la synsthèse directe de 3'-polynucléotides marqués
Publication
Application
Priority
US 59303196 A 19960129
Abstract (en)
[origin: EP0786468A2] The compounds of the invention are exemplified by the class of diglycolate synthesis supports particularly useful as support reagents for the direct synthesis of 3'-labeled polynucleotides. Generally, the compounds of the invention have the structure <CHEM> where T is an acid-cleavable hydroxyl protecting group, e.g., 4,4'-dimethoxytrityl; L1 is a linker for connecting a 3'-terminal nitrogen to carbon; L2 and L3 are linkers for connecting oxygen and carbon; W is a solid support, e.g., CPG or polystyrene; L4 is a linker for connecting the solid support to nitrogen; R1 and R2 are nitrogen substituents, e.g., hydrogen, lower alkyl, nitrogen protecting group, or label; and R3 through R7 are carbon substituents, e.g., hydrogen or lower alkyl. In a first particularly preferred embodiment, the synthesis supports of the invention are exemplified by compounds having the structure <CHEM> where DMT is 4,4'-dimethoxytrityl and W is polystyrene. In a second particularly preferred embodiment, the synthesis supports of the invention are exemplified by compounds having the structure <CHEM> where DMT and W are defined above.l
IPC 1-7
IPC 8 full level
G01N 33/58 (2006.01); C07H 1/00 (2006.01); C07H 21/00 (2006.01)
CPC (source: EP US)
C07H 21/00 (2013.01 - EP US); Y02P 20/55 (2015.11 - EP US); Y10S 435/81 (2013.01 - EP US)
Citation (examination)
- WO 9605215 A1 19960222 - PERKIN ELMER CORP [US]
- MULLAH ET AL: "Efficient Synthesis of doubledye-labeled oligodeoxyribonucleotide probes and their application in a real time PCR assay.", NUCLEIC ACIDS RESEARCH, vol. 26, no. 4, pages 1026 - 1031
Designated contracting state (EPC)
CH DE FR GB IT LI NL SE
DOCDB simple family (publication)
EP 0786468 A2 19970730; EP 0786468 A3 20000705; EP 0786468 B1 20041013; AU 1016997 A 19970828; AU 704180 B2 19990415; CA 2195256 A1 19970730; CA 2195256 C 20020528; DE 69731132 D1 20041118; DE 69731132 T2 20050908; JP 2008247908 A 20081016; JP 4127870 B2 20080730; JP H09208595 A 19970812; US 5736626 A 19980407
DOCDB simple family (application)
EP 97101131 A 19970124; AU 1016997 A 19970115; CA 2195256 A 19970116; DE 69731132 T 19970124; JP 2008094303 A 20080331; JP 2732697 A 19970128; US 59303196 A 19960129